干旱胁迫对柴胡皂苷合成途径关键酶基因表达的影响

发布时间：2018-11-13 10:10

【摘要】：目的:为了阐明在干旱胁迫下柴胡中的柴胡皂苷合成途径关键酶的基因表达及对皂苷含量的影响。方法:设置4个水平的土壤饱和含水量70%~80%、60%~70%、40%~50%、20%~30%,利用盆栽控水的方法栽培柴胡。采用RT-PCR方法检测6个月和1年生柴胡苗根的皂苷合成途径关键酶基因的表达,HPLC法测定柴胡皂苷a、d的含量。结果:4个柴胡皂苷合成途径关键酶HMGR(3-羟基-3-甲基-戊二酸单酰辅酶A还原酶)、IPPI(异戊烯基焦磷酸异构酶)、FPS(法尼基焦磷酸合酶)和β-AS(β-香树素合成酶)基因在土壤饱和含水量40%~50%时表达量最高,饱和含水量20%~30%时降到最低,皂苷含量的变化趋势与基因表达一致。结论:柴胡皂苷合成途径关键酶基因在适度干旱时能够显著提高表达量,从而间接提高皂苷的含量。
[Abstract]:Aim: to elucidate the gene expression of the key enzymes of saponins synthesis pathway in Bupleurum chinensis under drought stress and its effect on the content of saponins. Methods: four levels of saturated water content of soil were set up, and the water content of the soil was 70% and 70%, about 40% and 50%, respectively, and the water control method of pot culture was used to cultivate Bupleurum chinense. RT-PCR method was used to detect the expression of key enzyme genes of saponins synthesis pathway in the roots of 6-month-old and 1-year-old Bupleurum chinensis seedlings, and the content of saikosaponin Asad was determined by HPLC method. Results: four key enzymes of saikosaponin synthesis pathway HMGR (3-hydroxy-3-methyl-glutaryl coA reductase), IPPI (isopentenyl pyrophosphate isomerase). FPS (farinopyrophosphatase) and 尾 -vanillin synthase (尾 -vanillin synthase) genes expressed the highest amount at 40 ~ 50 saturated water content in soil and the lowest at 20 ~ 30% saturated water content. The change trend of saponin content was consistent with gene expression. Conclusion: the key enzyme genes of saikosaponin biosynthesis pathway can significantly increase the expression of saponins in moderate drought and thus indirectly increase the content of saponins.
【作者单位】：河北中医学院河北省涉县农牧局
【基金】：河北省高等学校科学技术研究项目(QN2015073) 河北省中医药管理局科研计划项目(2015081) 河北省现代农业产业技术体系中药材产业创新团队项目(7002017012013)
【分类号】：S567.79

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