清湿化瘀法对子宫腺肌病小鼠Ras基因和COX2-PGE2-P450arom正反馈环调控机制变化的研究

发布时间：2018-11-13 15:57

【摘要】：目的通过观察Ras基因表达蛋白和COX2—PGE2—P450arom正反馈环中三指标在子宫腺肌病小鼠治疗前后随动情周期变化的表达差异,及其各组子宫腺肌病小鼠自身在位及异位内膜上的表达差异,研究清湿化瘀法对子宫腺肌病小鼠可能的作用靶点,以探索清湿化瘀法治疗子宫腺肌病的作用机制。方法选取8周龄ICR雌鼠,通过异体垂体移植术建立小鼠子宫腺肌病模型,采用高、中、低剂量的内异康复片和孕三烯酮以及丹莪妇康煎膏分别给药治疗,灌胃治疗3月后取样,应用组织病理学检查、免疫组织化学染色法检测不同周期在位和异位内膜上的Ras和COX2、PGE2、P450arom的表达。结果 (1)手术操作过程对造模结果无影响(P0.01);(2)造模后与正常组相比,各组的在位和异位内膜Ras和COX2—PGE2—P450arom的表达均升高(P0.01),且6月模型组显著高于3月模型组(P0.01),说明Ras和COX2—PGE2—P450arom表达量在造模后随病程进展逐渐升高。(3)治疗前、后各模型组同期异位内膜上的Ras和COX2—PGE2—P450arom的表达量高于在位内膜(P0.01)。(4)治疗后,与6月模型组同期相比,内异康复片高、中计量组,孕三烯酮组,丹莪妇康煎膏组的小鼠在位和异位内膜的Ras和COX2—PGE2—P450arom的表达量均显著降低(P0.01)。结论清湿化瘀法可能通过下调在位及异位内膜Ras基因蛋白表达或抑制COX2—PGE2—P450arom正反馈作用而从某一条或多途径达到治疗子宫腺肌病的目的。
[Abstract]:Objective to observe the difference of expression of Ras gene expression protein and COX2-PGE2-P450arom positive feedback loop with estrous cycle before and after treatment in mice with adenomyosis. In order to explore the mechanism of removing dampness and removing blood stasis in treating adenomyosis of uterus, the possible targets of removing dampness and removing stasis were studied in order to explore the mechanism of removing dampness and removing blood stasis in the treatment of adenomyosis. Methods eight week-old ICR female rats were selected to establish the model of adenomyosis by allografts of pituitary gland in mice. The mice were treated with high, middle and low doses of Neihekangfu tablets, pregnenotrienone and Dan'e Fukang decoction respectively, and the samples were taken after 3 months of oral administration. The expression of Ras and COX2,PGE2,P450arom in different cycles of eutopic and ectopic endometrium were detected by histopathology and immunohistochemical staining. Results (1) the procedure of operation had no effect on the results of modeling (P0.01). (2) the expression of Ras and COX2-PGE2-P450arom in eutopic and ectopic endometrium of each group was higher than that of normal group (P0.01), and the expression of Ras and COX2-PGE2-P450arom in 6-month model group was significantly higher than that in 3-month model group (P0.01). The results showed that the expression of Ras and COX2-PGE2-P450arom increased with the course of the model. (3) before treatment, the expression of Ras and COX2-PGE2-P450arom on ectopic endometrium in each model group was higher than that in eutopic endometrium (P0.01). (4). Compared with the model group in June, the expression of Ras and COX2-PGE2-P450arom in the eutopic and ectopic endometrium of the mice in the middle dose group, pregnancy trienone group and Dan'e Fukang decoction group were significantly decreased (P0.01). Conclusion the method of removing dampness and removing blood stasis may be used to treat adenomyosis by down-regulating the expression of Ras gene in eutopic and ectopic endometrium or inhibiting the positive feedback of COX2-PGE2-P450arom.
【作者单位】：成都中医药大学;成都中医药大学附属医院;
【基金】：国家自然科学基金(No.81273796)
【分类号】：R285.5

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