转基因大豆MON89788基体标准物质的研制

发布时间：2018-11-13 16:33

【摘要】：转基因产品成分检测是转基因生物安全管理和标识的重要支撑,而转基因生物标准物质是获得准确、可靠、具有可比性检测结果的保证。转基因大豆(Glycine max)MON89788为我国批准进口用作加工原料的农业转基因生物,因此,制备转基因大豆MON89788基体标准物质是对其进行安全管理所必需的。本研究通过将转基因大豆MON89788和其受体材料A3244分别冷冻研磨成粉末,按质量分数称量配制,得到转基因含量为49.3 g/kg的标准物质。采用方差分析法(F检验法)进行均匀性检验,统计量F值为1.618,小于临界值F_((0.05,11,24)),标准物质均匀性良好;短期稳定性考察结果表明,标准物质可在常温下稳定储存4周,在-20℃下长期稳定性达到30个月。本批MON89788基体标准物质有两个特性量值,一是基于重量法配比混合的粉末质量分数,量值为49.3 g/kg,扩展不确定度为2.0 g/kg;二是基于q RT-PCR 8家实验室联合定值的转基因DNA与总DNA的质量分数,量值为49.8 ng/μg,扩展不确定度为5.9 ng/μg。本批标准物质每瓶1.0 g,使用时最小取样量为100 mg,可用于对转基因大豆MON89788进行定性和定量检测,以及转基因大豆MON89788特异性检测方法的评价和实验室质量控制。本研究为我国转基因检测基体标准物质的研制提供了理论基础和技术支持。
[Abstract]:Component detection of transgenic products is an important support for the safety management and marking of transgenic organisms, and the standard material of transgenic organisms is the guarantee of obtaining accurate, reliable and comparable test results. Genetically modified soybean (Glycine max) MON89788) is an agricultural transgenic organism approved by China to be used as raw material for processing. Therefore, the preparation of MON89788 matrix reference material is necessary for its safety management. In this study, the transgenic soybean MON89788 and its receptor material A3244 were ground into powder by freezing, and the standard material with transgenic content of 49.3 g/kg was obtained according to the weight fraction. Using the method of variance analysis (F test) to test the homogeneity, the statistic F value is 1.618, which is less than the critical value F _ (0.05 ~ 1124),). The results of short-term stability test showed that the standard materials could be stored steadily for 4 weeks at room temperature and 30 months at -20 鈩，

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