膨化全脂大豆对延边黄牛SCD基因表达及肌肉脂肪酸含量的影响

发布时间：2018-11-14 12:55

【摘要】：为了探讨饲喂膨化全脂大豆对延边黄牛肝脏、肌肉和脂肪组织中硬脂酰辅酶A去饱和酶(SCD)基因表达的影响及其与肌肉脂肪酸含量的相关性。选取健康、体重相近的延边黄牛阉牛8头,随机分为两个处理组,每组4头,对照组饲喂全混合日粮,试验组用膨化全脂大豆替代全混合日粮中的豆粕,并保证日粮营养水平不变,试验期为90 d。试验结束后屠宰,采集肝脏、肌肉、脂肪组织,检测SCD基因的表达量,并与肌肉脂肪酸含量进行相关分析。结果表明:SCD基因在肌肉中的表达量试验组极显著高于对照组(P0.01);在肝脏和脂肪中的表达量试验组低于对照组,但差异不显著(P0.05);SCD基因表达量与肌肉中棕榈油酸(C161)含量呈显著正相关(P0.05),相关系数为0.999;与油酸(C181)含量呈极显著正相关(P0.01),相关系数为0.995;与亚油酸(C182)含量呈显著正相关(P0.05),相关系数为0.975。膨化全脂大豆替代全混合日粮中的豆粕增加了SCD基因在肌肉中的表达量,能够改变牛肉脂肪酸组成,使牛肉富含有益人体的不饱和脂肪酸。
[Abstract]:To investigate the effect of full fat soybean feeding on the expression of stearyl CoA desaturase (SCD) gene in liver, muscle and adipose tissue of Yanbian Yellow cattle and its correlation with muscle fatty acid content. Eight healthy Yanbian cattle castrated cattle of similar body weight were randomly divided into two treatment groups (4 in each group). The control group was fed with total mixed diet and the experimental group was fed with expanded whole fat soybean instead of soybean meal in the whole mixed diet. The nutrition level of the diet was not changed, and the trial period was 90 days. After slaughter, liver, muscle and adipose tissue were collected, and the expression of SCD gene was detected, and the correlation between the expression of SCD gene and fatty acid content in muscle was analyzed. The results showed that the expression of SCD gene in the muscle was significantly higher in the experimental group than in the control group (P0.01), but the expression level in liver and fat was lower in the experimental group than in the control group, but the difference was not significant (P0.05). The expression of SCD gene was positively correlated with the content of palmitic acid (C161) in muscle (P0.05) and with the content of oleic acid (C181) (P 0.05), and with the content of oleic acid (C181) (P 0.01). There was a significant positive correlation between the content of linoleic acid (C182) and the content of linoleic acid (P0.05), the correlation coefficient was 0.975. The expanded whole fat soybean instead of soybean meal in the whole mixed diet increased the expression of SCD gene in the muscle and changed the composition of beef fatty acids, which made the beef rich in unsaturated fatty acids that are beneficial to human body.
【作者单位】：延边大学农学院;
【基金】：吉林省重点科技攻关项目[20160204017NY] 吉林省现代肉牛产业技术体系建设研究课题[201635]
【分类号】：S823.5

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