肺腺癌胸水细胞块EGFR基因突变检测分析
发布时间：2018-11-15 19:41
【摘要】：目的探讨肺腺癌胸水细胞块中表皮生长因子受体(epidermal growth factor receptor,EGFR)基因突变检测的临床价值。方法采用ARMS-PCR法检测143例肺腺癌细胞块和269例肺腺癌组织块中EGFR的29种突变类型,并检测细胞块同时送检组织块的患者49例的一致性。结果细胞块EGFR突变型71例,阳性率49.65%(71/143);组织块EGFR突变型131例,阳性率44.26%(131/269);49例有组织块对照的细胞块EGFR结果一致性有36例,一致率达73.47%(36/49),其中细胞块EGFR的阳性率44.90%(22/49),组织块阳性率67.34%(33/49)。结论肺腺癌胸水细胞块EGFR的阳性率略高于组织块;有恶性胸水的肺腺癌患者原发灶组织发生EGFR突变的概率较高。
[Abstract]:Objective to investigate the clinical value of epidermal growth factor receptor (epidermal growth factor receptor,EGFR) gene mutation in pleural effusion of lung adenocarcinoma. Methods ARMS-PCR method was used to detect 29 mutation types of EGFR in 143 cases of lung adenocarcinoma cells and 269 cases of lung adenocarcinoma tissue mass. Results the positive rate of EGFR mutation was 49.65% (71 / 143) and EGFR mutation was 44.26% (131 / 269). The results of EGFR were consistent in 36 cases (73.47%), in which the positive rate of EGFR was 44.90% (22 / 49) and the positive rate of tissue mass was 67.34% (33 / 49). Conclusion the positive rate of EGFR in pleural effusion cell mass of lung adenocarcinoma is slightly higher than that in tissue mass, and the probability of EGFR mutation in primary tissues of lung adenocarcinoma with malignant pleural effusion is higher.
【作者单位】： 第三军医大学大坪医院野战外科研究所;
【分类号】：R734.2
[Abstract]:Objective to investigate the clinical value of epidermal growth factor receptor (epidermal growth factor receptor,EGFR) gene mutation in pleural effusion of lung adenocarcinoma. Methods ARMS-PCR method was used to detect 29 mutation types of EGFR in 143 cases of lung adenocarcinoma cells and 269 cases of lung adenocarcinoma tissue mass. Results the positive rate of EGFR mutation was 49.65% (71 / 143) and EGFR mutation was 44.26% (131 / 269). The results of EGFR were consistent in 36 cases (73.47%), in which the positive rate of EGFR was 44.90% (22 / 49) and the positive rate of tissue mass was 67.34% (33 / 49). Conclusion the positive rate of EGFR in pleural effusion cell mass of lung adenocarcinoma is slightly higher than that in tissue mass, and the probability of EGFR mutation in primary tissues of lung adenocarcinoma with malignant pleural effusion is higher.
【作者单位】： 第三军医大学大坪医院野战外科研究所;
【分类号】：R734.2
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