水牛6个Y染色体基因的拷贝数变异研究
发布时间：2018-11-16 08:14
【摘要】：目前,牛Y染色体转录组学分析表明至少有6种编码蛋白的多拷贝基因位于Y染色体雄性特异区(MSY)。牛MSY基因的拷贝数变异与雄性繁殖力和精子发生机制相关。由于目前没有水牛全基因组序列,国内外仅研究了水牛Y染色体上TSPY基因的拷贝数变异。关于Y染色体多拷贝基因拷贝数变异研究,主要集中在人类、牛及小鼠的TSPY、HSFY及PRAMEY等基因,没有关于水牛6个Y染色体基因HSFY、ZNF280BY、SRY、DBY、UTY和OFD1Y拷贝数变异的报道。因此,本研究使用实时定量PCR(q PCR)方法,检测3个Y染色体基因SRY、HSFY和ZNF280BY在16个中国沼泽型水牛品种、外国沼泽型水牛(越南水牛和老挝水牛)及江河型水牛(摩拉水牛和尼里拉菲水牛)共计302头公水牛中的拷贝数变异,以及3个Y染色体基因DBY、UTY和OFD1Y在6个水牛品种105头公水牛中的拷贝数变异,获得如下创新性结果:1.首次揭示水牛6个Y染色体基因(HSFY、ZNF280BY、SRY、DBY、UTY和OFD1Y基因)的拷贝数变异,发现HSFY,ZNF280BY和DBY基因的拷贝数中值数(变化范围)分别为48(20-145),257(73-974)和2(1-4);SRY和OFD1Y基因的拷贝数中值数(变化范围)为1(1-4),在越南水牛中拷贝数表现出差异;UTY基因拷贝数为1。2.揭示HSFY和ZNF280BY基因在18个水牛品种中为多拷贝基因,且拷贝数在不同个体和品种中有很大差异;DBY基因在6个水牛品种中为低拷贝基因;SRY、OFD1Y和UTY基因在中国水牛品种中为单拷贝基因。3.发现HSFY、ZNF280BY、SRY、DBY、UTY和OFD1Y基因的拷贝数变异在沼泽型水牛和江河型水牛中没有显著差异。
[Abstract]:At present, transcriptome analysis of bovine Y chromosome indicates that at least 6 multicopy genes of protein are located in the male specific region of Y chromosome (MSY). The copy number variation of bovine MSY gene is related to male reproduction and spermatogenesis. Because there is no buffalo genome sequence at present, only the copy number variation of TSPY gene on buffalo Y chromosome has been studied at home and abroad. The study on the copy number variation of Y chromosome multiple copy genes is mainly focused on the TSPY,HSFY and PRAMEY genes of human, cattle and mice. There is no report on the HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y copy number variation of six Y chromosome genes in buffalo. Therefore, real-time quantitative PCR (q PCR) was used to detect three Y chromosome genes SRY,HSFY and ZNF280BY in 16 Chinese marsh buffalo breeds. Copy number variation and DBY, of three Y chromosome genes in 302 foreign swamp buffalo (Vietnamese buffalo and Lao buffalo) and river water buffalo (Mora buffalo and Nilirafi buffalo) The copy number variation of UTY and OFD1Y in 105 male buffalo breeds was as follows: 1. The variation of copy number of six Y chromosome genes (HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y) in buffalo was revealed for the first time. It was found that the median copy number (range of variation) of HSFY,ZNF280BY and DBY genes was 48 (20-145), respectively. 257 (73-974) and 2 (1-4); The median copy number (range of variation) of SRY and OFD1Y gene was 1 (1-4), which was different in Vietnamese buffalo, and the copy number of UTY gene was 1.2. The results showed that HSFY and ZNF280BY genes were multi-copy genes in 18 buffalo breeds, and the number of copies was different in different individuals and varieties, and DBY gene was low copy gene in 6 buffalo breeds. SRY,OFD1Y and UTY genes are single copy genes in Chinese buffalo breeds. It was found that there was no significant difference in copy number variation of HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y gene between swamp buffalo and river buffalo.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S823.83
本文编号：2334978
[Abstract]:At present, transcriptome analysis of bovine Y chromosome indicates that at least 6 multicopy genes of protein are located in the male specific region of Y chromosome (MSY). The copy number variation of bovine MSY gene is related to male reproduction and spermatogenesis. Because there is no buffalo genome sequence at present, only the copy number variation of TSPY gene on buffalo Y chromosome has been studied at home and abroad. The study on the copy number variation of Y chromosome multiple copy genes is mainly focused on the TSPY,HSFY and PRAMEY genes of human, cattle and mice. There is no report on the HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y copy number variation of six Y chromosome genes in buffalo. Therefore, real-time quantitative PCR (q PCR) was used to detect three Y chromosome genes SRY,HSFY and ZNF280BY in 16 Chinese marsh buffalo breeds. Copy number variation and DBY, of three Y chromosome genes in 302 foreign swamp buffalo (Vietnamese buffalo and Lao buffalo) and river water buffalo (Mora buffalo and Nilirafi buffalo) The copy number variation of UTY and OFD1Y in 105 male buffalo breeds was as follows: 1. The variation of copy number of six Y chromosome genes (HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y) in buffalo was revealed for the first time. It was found that the median copy number (range of variation) of HSFY,ZNF280BY and DBY genes was 48 (20-145), respectively. 257 (73-974) and 2 (1-4); The median copy number (range of variation) of SRY and OFD1Y gene was 1 (1-4), which was different in Vietnamese buffalo, and the copy number of UTY gene was 1.2. The results showed that HSFY and ZNF280BY genes were multi-copy genes in 18 buffalo breeds, and the number of copies was different in different individuals and varieties, and DBY gene was low copy gene in 6 buffalo breeds. SRY,OFD1Y and UTY genes are single copy genes in Chinese buffalo breeds. It was found that there was no significant difference in copy number variation of HSFY,ZNF280BY,SRY,DBY,UTY and OFD1Y gene between swamp buffalo and river buffalo.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S823.83
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