中国部分地区对虾白斑综合征病毒胶原蛋白基因缺失变异分析

发布时间：2018-11-16 11:25

【摘要】：为了解我国主要对虾养殖区的白斑综合征病毒(WSSV)胶原蛋白基因(wsv001)蛋白结构变异情况,以2015年13个省市发病地区采集的57份WSSV阳性样品为模板,用特异引物对目的片段进行扩增,连接转化扩增片段并进行测序分析。结果显示,57份WSSV阳性样品中,扩增出现条带的样品共有18份。氨基酸序列比对结果显示,扩增的wsv001片段所编码氨基酸出现三类变异,包括18个氨基酸小片段缺失,121个氨基酸大片段缺失及氨基酸插入,此外,还有8个氨基酸存在突变。结果表明,我国不同地区wsv001基因及其编码氨基酸存在明显缺失变异。对wsv001蛋白结构缺失变异的分析,为进一步对其蛋白功能和WSSV毒株毒力及WSSV对环境适应能力的研究提供参考。
[Abstract]:In order to understand the structural variation of (WSSV) collagen gene (wsv001) of white spot syndrome virus (WSSV) in main shrimp culture areas in China, 57 WSSV positive samples collected from 13 provinces and cities in 2015 were used as template. The target fragment was amplified with specific primers, ligated and transformed into the amplified fragment and sequenced. The results showed that 18 of 57 WSSV positive samples were amplified. The results of amino acid sequence alignment showed that there were three kinds of variations in amino acids encoded by the amplified wsv001 fragment, including 18 small amino acid deletion, 121 large amino acid deletion and amino acid insertion, in addition, there were 8 amino acid mutations. The results showed that the wsv001 gene and its encoded amino acids in different regions of China were significantly deleted and mutated. The analysis of structural deletion of wsv001 protein provides a reference for further study on its protein function, virulence of WSSV strain and adaptability of WSSV to environment.
【作者单位】：农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所;上海海洋大学水产与生命学院;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室;
【基金】：国家重点基础研究发展计划(2012CB114401) 农业部948项目(2016-X56)
【分类号】：S945.4

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