胡杨F-box基因克隆和功能分析

发布时间：2018-11-16 19:30

【摘要】：F-box蛋白在泛素-蛋白酶体途径中能够特异识别底物蛋白,调节多种激素的信号转导途径,并响应多种非生物胁迫,参与植物抗逆途径。胡杨(Populus euphratica)是中国西北沙漠唯一能成林的高大乔木树种,对盐碱、干旱、极端温度等抵抗能力较强,是研究林木抗逆机理的候选模式树种。本研究对胡杨耐盐性状关联分析获得的F-box基因进行克隆、表达特性分析、亚细胞定位和转化胡杨原生质体的耐盐性测定,旨在研究胡杨F-box基因的功能特性。胡杨F-box基因全长1 086 bp,编码361个氨基酸,含有一个F-box domain和一个F-box associated domain。q RT-PCR表明胡杨F-box基因在各组织中均有表达,无组织特异性,其表达受盐、高温、低温、干旱和ABA胁迫诱导。PEG介导的胡杨原生质体瞬时表达发现F-box蛋白定位于细胞核上,流式细胞术证实过表达F-box基因能够提高胡杨原生质体的耐盐性。研究初步揭示了胡杨F-box基因的表达特性和耐盐性,为进一步解析胡杨F-box基因的功能及胡杨抗逆分子机理提供帮助。
[Abstract]:F-box protein can specifically recognize substrate proteins, regulate the signal transduction pathways of many hormones in the ubiquitin proteasome pathway, and respond to various abiotic stresses and participate in plant stress resistance pathway. Populus euphratica (Populus euphratica) is the only tall tree species in northwest desert of China, which has strong resistance to salt and alkali, drought and extreme temperature, and is a candidate tree species for studying the mechanism of forest stress resistance. In order to study the functional characteristics of F-box gene of Populus euphratica, the F-box gene obtained from salt-tolerance correlation analysis was cloned, expressed, and transformed into protoplasts of Populus euphratica. The total length of F-box gene of Populus euphratica is 1 086 bp, encoding 361 amino acids, which contains a F-box domain and a F-box associated domain.q RT-PCR indicating that the F-box gene of Populus euphratica is expressed in all tissues without tissue specificity, and its expression is salt and high temperature. The transient expression of PEG mediated protoplast of Populus euphratica showed that the F-box protein was located on the nucleus, and the overexpression of F-box gene could improve the salt-tolerance of Populus euphratica protoplast by flow cytometry. The study revealed the characteristics of F-box gene expression and salt tolerance of Populus euphratica, which could help to further analyze the function of F-box gene and the molecular mechanism of stress resistance of Populus euphratica.
【作者单位】：西北农林科技大学林学院;林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所;
【基金】：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014ZX001-4) 林业公益性行业科研专项经费(201404101) 新疆自治区高层次人才引进工程(2014)共同资助
【分类号】：Q943.2;S792.11

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