松墨天牛Homothorax基因的鉴定及表达分析

发布时间：2018-11-17 06:34

【摘要】：为了探讨Meis家族中Homothorax基因在昆虫中的表达特性,以松墨天牛为研究对象,从cDNA文库中筛选到松墨天牛Homothorax基因,命名为MaHth(GenBank:KX364396)。该序列长为1347bp,编码448个氨基酸。由此预测的蛋白质二级结构主要由α螺旋与无规则卷曲组成,其次是β转角与延伸链;SWISS-MODEL同源建模预测其三级结构的基元为α螺旋-β转角-α螺旋。MaHth基因编码蛋白,定位于细胞核,并存在2个独立的核定位信号:KRDK、KKNQKKR。通过DNAMAN软件比对发现MaHth与赤拟谷盗同源性最高,为97%,且存在Hox保守结构域序列;用MEGA4.0构建系统发育树,显示松墨天牛与赤拟谷盗处在同一分支。通过RT-qPCR分析表明:卵、蛹中的表达量比幼虫、成虫中的高,在成虫中最低;幼虫头部中MaHth表达量最高,马氏管中表达量较低;成虫头部、马氏管中MaHth表达量最高,足、翅、触角中表达量较低。
[Abstract]:In order to investigate the expression characteristics of Homothorax gene in Meis family, the Homothorax gene was selected from cDNA library and named MaHth (GenBank:KX364396). The sequence is 1347 BP and encodes 448 amino acids. The protein secondary structure was mainly composed of 伪 helix and irregular crimp, followed by 尾 rotation angle and extension chain. SWISS-MODEL homology model predicts that the tertiary structure of SWISS-MODEL is 伪 helix-尾 angular 伪 helix. MaHth gene encodes protein and is located in the nucleus, and there are two independent nuclear localization signals: KRDK,KKNQKKR.. By comparing with DNAMAN software, it was found that MaHth had the highest homology (97%) with Hox conserved domain sequence, and the phylogenetic tree was constructed by MEGA4.0, which showed that the two species were in the same branch. The results of RT-qPCR analysis showed that the expression of MaHth in eggs and pupae was higher than that in larva and in adult, the expression of MaHth in larval head was the highest, and that in Markov tube was lower. In the head of adult worm, the expression of MaHth was the highest in the Markov tube, and lower in the feet, wings and antennae.
【作者单位】：华南农业大学林学与风景园林学院;
【基金】：国家自然科学基金项目(31470653) 广东省自然科学基金项目(2015A030313416)
【分类号】：S763.38

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