银鲳ghr和igfbp1基因表达特征、组织分布及操作胁迫对其的影响（英文）

发布时间：2018-11-18 14:51

【摘要】：为探讨急性操作胁迫对银鲳生长的影响,首次克隆了3种生长调控相关基因-生长激素受体1(ghr1)、生长激素受体2(ghr2)和胰岛素样受体1(igfbp1),并对3种基因在银鲳肝脏、心脏、性腺、肌肉和肾脏等不同组织中的表达进行了比较。定量表达结果表明3种基因均广泛分布于所研究的各个组织中,而其中肾脏、性腺和心脏中的表达量较其它组织低。在银鲳受到外界操作胁迫刺激后,3种基因呈现不同的表达模式:其中ghr2和igfbp1的表达下调,而ghr2的表达量与对照组无显著性差异。结果表明,ghr2和igfbp1在银鲳生长调节中起到重要的作用;在胁迫环境中,外界刺激可通过下调生长相关基因表达以影响鱼体的生长。
[Abstract]:In order to investigate the effect of acute operation stress on the growth of Pomfret, three growth regulation-related genes, growth hormone receptor 1 (ghr1), growth hormone receptor 2 (ghr2) and insulin-like receptor 1 (igfbp1), were cloned for the first time. The expression of three genes in liver, heart, gonad, muscle and kidney were compared. The results of quantitative expression showed that the three genes were widely distributed in all tissues studied, but the expression levels in kidney, gonad and heart were lower than those in other tissues. After being stimulated by external operating stress, the three genes showed different expression patterns: the expression of ghr2 and igfbp1 was down-regulated, but the expression of ghr2 was not significantly different from that of the control group. The results showed that ghr2 and igfbp1 played an important role in the growth regulation of Pomfret, and in the stress environment, the external stimulation could affect the growth of fish by down-regulating the expression of growth-related genes.
【作者单位】：中国水产科学研究院东海水产研究所;
【基金】：中央级公益性科研院所基本科研业务费项目(东2014Y01) 上海市自然基金项目(No.12ZR1455000) 国家自然基金项目(No.41306170)
【分类号】：S917.4

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