蝴蝶兰PhalPI基因的克隆及在花器官突变体中的表达分析
发布时间：2018-11-18 14:53
【摘要】：为深入研究兰科植物花器官发育的调控机理,从蝴蝶兰花瓣中克隆了一个B类MADS-box转录因子PhalPI(GenBank登录号为KY020416)。序列分析表明,该基因的cDNA全长为944 bp,含完整的开放阅读框,可编码210个氨基酸,属于BGLO/PI蛋白家族,与蝴蝶兰属的PhPI10和PeMADS6基因关系最近;表达模式分析表明,PhalPI基因在生殖器官中表达,在营养器官中不表达,在授粉后的子房中,该基因的表达水平降低。在5种花器官突变体中,PhalPI基因在萼片唇瓣化突变体的萼片和蕊柱中表达水平明显升高;在雄蕊花瓣化突变体的萼片和侧瓣中表达水平降低,在其唇瓣和蕊柱中显著升高;在侧瓣合柱化突变体的蕊柱中,PhalPI基因的表达也发生了显著升高;PhalPI基因表达的改变与花器官形态的突变相关;而在侧瓣唇瓣化和侧瓣花药化突变体中,PhalPI基因的表达水平没有变化。推测该基因在决定蝴蝶兰侧瓣和唇瓣的发育中起重要的调控作用。
[Abstract]:In order to study the regulation mechanism of flower organ development in orchids, a class B MADS-box transcription factor (PhalPI (GenBank) was cloned from the petals of Phalaenopsis (PhalPI (GenBank accession number is KY020416). Sequence analysis showed that the cDNA length of the gene was 944 bp, with a complete open reading frame, encoding 210 amino acids, belonging to the BGLO/PI protein family, and most closely related to the PhPI10 and PeMADS6 genes of Phalaenopsis. Expression pattern analysis showed that PhalPI gene was expressed in reproductive organs, but not in vegetative organs, but decreased in ovary after pollination. In the five floral mutants, the expression level of PhalPI gene was significantly increased in sepals and stamens, but decreased in the sepals and lateral petal mutants of stamen petal mutants. The expression of PhalPI gene was also significantly increased in the column of lateral valve column mutants, and the change of PhalPI gene expression was related to the mutation of floral organ morphology. However, there was no change in the expression level of PhalPI gene in the lateral valvular and lateral valve anther mutants. It is speculated that this gene plays an important role in determining the development of lateral and labial valves of Phalaenopsis.
【作者单位】： 郑州师范学院生物工程研究所;河南农业大学生命科学学院;
【基金】：河南省科技攻关项目(162102110073)~~
【分类号】：S682.31
，
本文编号：2340346
[Abstract]:In order to study the regulation mechanism of flower organ development in orchids, a class B MADS-box transcription factor (PhalPI (GenBank) was cloned from the petals of Phalaenopsis (PhalPI (GenBank accession number is KY020416). Sequence analysis showed that the cDNA length of the gene was 944 bp, with a complete open reading frame, encoding 210 amino acids, belonging to the BGLO/PI protein family, and most closely related to the PhPI10 and PeMADS6 genes of Phalaenopsis. Expression pattern analysis showed that PhalPI gene was expressed in reproductive organs, but not in vegetative organs, but decreased in ovary after pollination. In the five floral mutants, the expression level of PhalPI gene was significantly increased in sepals and stamens, but decreased in the sepals and lateral petal mutants of stamen petal mutants. The expression of PhalPI gene was also significantly increased in the column of lateral valve column mutants, and the change of PhalPI gene expression was related to the mutation of floral organ morphology. However, there was no change in the expression level of PhalPI gene in the lateral valvular and lateral valve anther mutants. It is speculated that this gene plays an important role in determining the development of lateral and labial valves of Phalaenopsis.
【作者单位】： 郑州师范学院生物工程研究所;河南农业大学生命科学学院;
【基金】：河南省科技攻关项目(162102110073)~~
【分类号】：S682.31
，
本文编号：2340346
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