陕西省水稻Wx基因种质资源遗传多样性分析

发布时间：2018-11-18 17:57

【摘要】：稻米品质与稻米中直链淀粉含量(AC)有关,而直链淀粉含量与蜡质基因(Wx)的基因型有关,研究表明Wx基因第一内含子的剪接效率决定直链淀粉含量。明确陕西省主要水稻种质资源中Wx基因的基因型,可为水稻品质育种提供依据。应用CAPS法对112份陕西省水稻主要种质资源Wx基因第一内含子供体+1位碱基G/T进行检测,根据G/T碱基将112份供试材料的Wx基因分为GG、GT、TT等3种基因型。同时,按国标检测3种不同G/T型37份稻米的直链淀粉含量及其他相关性状。结果显示,112份供试材料中有1份材料为GG型,约占1%;25份为GT型,占22%;86份材料为TT型,约占77%。说明Wx基因第一内含子供体+1位碱基G/T的多态性与AC含量有良好的对应关系,与垩白粒率和垩白度有一定的对应关系。
[Abstract]:The quality of rice was related to amylose content (AC), while amylose content was related to the genotype of waxy gene (Wx). The results showed that the splicing efficiency of the first intron of Wx gene determined the amylose content. The genotypes of Wx gene in the main rice germplasm resources in Shaanxi Province can provide basis for rice quality breeding. The first intron donor of Wx gene in 112 rice germplasm in Shaanxi Province was detected by CAPS method. According to the G / T base, the Wx gene of 112 rice germplasm was divided into three genotypes, GG,GT,TT and so on. At the same time, amylose content and other related characters of 37 rice varieties with different G / T type were detected according to national standard. The results showed that one of the 112 tested materials was of GG type, 25 of them were GT type, 22 of them were TT type and about 7777. The results indicated that the polymorphism of G / T in the donor of the first intron of Wx gene had a good correspondence with AC content, and had a certain correspondence with chalky grain percentage and chalkiness.
【作者单位】：陕西理工学院生物科学与工程学院;陕西省水稻研究所;
【基金】：陕西省科技厅农业科技攻关项目(编号:2013K02-10-01);陕西省科技厅农业科技创新项目(编号:NKC01-01) 陕西省科技统筹创新工程计划(编号:2011KTCG02-01)
【分类号】：S511

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