阿魏酸对阿尔茨海默病转基因小鼠脑内氧化应激和凋亡相关蛋白的影响

发布时间：2018-11-19 13:58

【摘要】：本文主要研究阿魏酸对APP/PS1转基因小鼠脑内氧化应激和凋亡相关蛋白的影响。应用Western blot和Real-Time PCR方法检测野生型小鼠、APP/PS1转基因小鼠及不同浓度阿魏酸处理组小鼠脑内凋亡相关蛋白的表达情况,并测定各组小鼠脑内SOD活力和MDA含量。阿魏酸处理后的AD模型小鼠SOD与AD组相比活性增加,MDA含量减少,其中中浓度阿魏酸处理组的效果最为明显;APP/PS1转基因小鼠脑内所检测的凋亡相关蛋白p-JNK、p-C-Jun、Caspase-3、Bax和Bcl-2表达较野生型小鼠明显升高,阿魏酸处理后这些磷酸化蛋白的表达显著减少,并且中浓度阿魏酸处理组的效果最为明显。以上结果表明阿魏酸通过其强大的抗氧化作用降低AD小鼠脑内的氧化应激效应,并且可以降低AD小鼠脑内凋亡相关蛋白的表达,提示阿魏酸在AD的临床治疗方面具有重要的理论意义。
[Abstract]:The effects of ferulic acid on oxidative stress and apoptosis-related proteins in brain of APP/PS1 transgenic mice were studied. Western blot and Real-Time PCR methods were used to detect the expression of apoptosis-related proteins in the brain of wild type mice, APP/PS1 transgenic mice and ferulic acid treated mice with different concentrations, and the activity of SOD and the content of MDA in the brain of each group were measured. Compared with AD group, the activity of SOD was increased and the content of MDA was decreased in AD model mice treated with ferulic acid, among which the effect of ferulic acid treatment was the most obvious. Compared with wild-type mice, the expression of p-JNK-p-C-Jun-Caspase-3 Bax and Bcl-2 in the brain of APP/PS1 transgenic mice was significantly higher than that of wild-type mice, and the expression of these phosphorylated proteins was significantly decreased after ferulic acid treatment. The effect of medium concentration ferulic acid treatment was the most obvious. These results suggest that ferulic acid can reduce oxidative stress in brain of AD mice and decrease the expression of apoptosis-related protein in brain of AD mice. The results suggest that ferulic acid has important theoretical significance in the clinical treatment of AD.
【作者单位】：辽宁中医药大学;
【基金】：国家自然科学基金(81373718)
【分类号】：R285.5

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