擎天凤梨SAMs基因的分离及开花期的表达分析

发布时间：2018-11-26 18:34

【摘要】：为了获得擎天凤梨SAMs基因,明确其在乙烯生物合成中的作用,本试验在前期构建全长c DNA文库、批量EST测序的基础上,对目标单克隆进行Primer Walking测序获得了2个SAMs基因,分别命名为SAMs1和SAMs2。SAMs1基因(Gen Bank:KF787113)序列全长1 375bp,开放阅读框1 086 bp,编码一条361个氨基酸的序列;SAMs2基因(Gen Bank:KF787114)序列全长1 235bp,开放阅读框945 bp,编码一条314个氨基酸的序列。应用生物信息学方法对2个基因编码蛋白的氨基酸组成、理化性质、保守结构域、二级结构、三级晶体结构等方面进行了预测和分析。分子系统进化分析表明,SAMs1与禾本科植物的SAMs基因聚为一类,与SAMs2关系较远。对2个基因在开花期的表达变化以及乙烯释放量变化分析表明,SAMs1基因表达与内源乙烯的释放水平变化趋势一致,即在完全呈色的红色苞片中含量最高,其次是绿色苞片,最后为绿色叶片。推测SAMs1在乙烯的生物合成中起重要作用,SAMs2参与了除乙烯生物合成外的其它SAM生物代谢过程。本试验结果对研究擎天凤梨的乙烯生物合成、苞片呈色机理以及催花应用具有重要意义。
[Abstract]:In order to obtain SAMs gene and clarify its role in ethylene biosynthesis, two SAMs genes were obtained by Primer Walking sequencing on the basis of the construction of full-length c DNA library and batch EST sequencing. They were named SAMs1 and SAMs2.SAMs1 gene (Gen Bank:KF787113), respectively. The open reading frame 1086 bp, encodes a 361 amino acid sequence. The sequence of SAMs2 gene (Gen Bank:KF787114) is 1 235 BP, and open reading frame 945 bp, encodes a 314 amino acid sequence. The amino acid composition, physicochemical properties, conserved domain, secondary structure and tertiary crystal structure of two gene encoded proteins were predicted and analyzed by bioinformatics. Molecular phylogenetic analysis showed that SAMs1 was closely related to SAMs gene of Gramineae plants and had a far relationship with SAMs2. The analysis of the expression changes of two genes at anthesis and ethylene release showed that the SAMs1 gene expression was consistent with the change trend of endogenous ethylene release level, that is, the content of SAMs1 gene was the highest in the completely colored red bracts, followed by the green bracts. Finally, green leaves. It is suggested that SAMs1 plays an important role in ethylene biosynthesis, and SAMs2 is involved in the biosynthesis of SAM except ethylene biosynthesis. The results of this experiment are of great significance for the study of ethylene biosynthesis, the color mechanism of bracts and the application of flower promotion.
【作者单位】：浙江省萧山棉麻研究所;
【基金】：浙江省自然科学基金(LQ13C150002) 浙江省公益性技术应用研究计划项目(2012C22085)
【分类号】：S682.3

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