巴西橡胶树HbCCoAOMT基因克隆及其表达分析

发布时间：2018-11-27 14:11

【摘要】：木质素是植物细胞壁的成分之一,其含量高低影响到细胞壁的弹性。橡胶树乳管膨压是割胶后胶乳排出的初动力,乳管细胞壁的物理性能可能是影响乳管膨压的因素之一。本研究采用QRT-PCR和RACE技术,从巴西橡胶树无性系CATAS7-33-97的乳管细胞中克隆到一个木质素合成关键酶CCo AOMT基因的同源基因,命名为HbCCoAOMT。该基因的开放阅读框为741 bp,编码1个由246个氨基酸残基组成的蛋白质。该蛋白质分子量为27.76 ku,理论等电点为5.255。实时荧光定量PCR结果表明,割胶和乙烯利处理均下调HbCCoAOMT基因在胶乳中的表达。该基因的表达量以及内层树皮的木质素含量在两个橡胶树品种(CATAS8-79和PR107)间存在显著差异。HbCCoAOMT差异表达可能影响到乳管细胞木质素含量和乳管细胞壁的物理性能。
[Abstract]:Lignin is one of the components of plant cell wall, and its content affects the elasticity of cell wall. The pressure of rubber latex tube is the initial driving force of latex ejection after tapping, and the physical properties of the cell wall of latex tube may be one of the factors affecting the pressure of latex tube expansion. In this study, QRT-PCR and RACE techniques were used to clone a homologous gene of CCo AOMT gene, a key enzyme of lignin biosynthesis, from the latex tube cells of CATAS7-33-97 clone of Hevea brasiliensis, named HbCCoAOMT.. The open reading frame of the gene encodes a protein composed of 246 amino acid residues for 741 bp,. The molecular weight of the protein is 27.76 ku, and the theoretical isoelectric point is 5.255. The results of real-time fluorescence quantitative PCR showed that both tapping and ethephon treatment could down-regulate the expression of HbCCoAOMT gene in latex. There were significant differences in the expression of the gene and the lignin content in the inner bark between the two Hevea varieties (CATAS8-79 and PR107). The differential expression of HbCCoAOMT might affect the lignin content and the physical properties of the cell wall of milk tube.
【作者单位】：中国热带农业科学院橡胶研究所;
【基金】：现代农业产业技术体系建设专项资金(No.CARS-34-GW1)
【分类号】：S794.1

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