陆川猪FAM134B基因克隆及生物信息学分析

发布时间：2018-11-28 20:56

【摘要】：本试验旨在对陆川猪FAM134B基因进行克隆及生物信息学分析。本研究利用Gen Bank公布的猪序列设计引物,应用RT-PCR扩增得到目的基因片段,应用生物信息学方法分析和预测了陆川猪FAM134B基因的理化性质与二级结构。结果显示:陆川猪FAM134B基因编码区全长1 107 bp,编码368个氨基酸;陆川猪FAM134B基因与与金华猪(JX854456.1)、牛(KM587693.1)、山羊(KF684947.1)、人(NM_001034850.2)、小鼠(NM_001034851.2)、大鼠(NM_001034912.1)、非洲爪蟾(NM_001126975.1)序列相似性分别为87.80%、87.44%、86.9%、68.47%、67.01%、67.29%、61.44%,结合系统进化树分析,结果表明不同物种FAM134B基因在进化过程中具有高度保守性。通过比较分析发现,陆川猪FAM134B基因CDS序列区比NCBI公布的金华猪FAM134B序列(JX854456.1)多了一段129 bp序列,比预测的猪FAM134B序列(XM_003483804.2)少了一段371 bp,这提示所扩增得到的陆川猪FAM134B基因有可能是另外一种剪接体。本研究成功克隆陆川猪FAM134B基因完整的CDS序列,为今后FAM134B基因在陆川猪的脂肪沉积及脂肪代谢方面的研究提供基础理论。
[Abstract]:The aim of this study was to clone and bioinformatics analysis of FAM134B gene in Luchuan pig. In this study, primer was designed by using pig sequence published by Gen Bank and the target gene fragment was amplified by RT-PCR. The physicochemical properties and secondary structure of FAM134B gene of Luchuan pig were analyzed and predicted by bioinformatics method. The results showed that the encoding region of FAM134B gene of Luchuan pig was 1 107 bp, encoding 368 amino acids. The association of FAM134B gene with Jinhua pig (JX854456.1), cattle (KM587693.1), goat (KF684947.1), human (NM_001034850.2), mouse (NM_001034851.2), and rat (NM_001034912.1), and the relationship between Luchuan pig FAM134B gene and Jinhua pig (JX854456.1), cattle (KM587693.1), goat (KF684947.1), human (NM_001034850.2), mouse (NM_001034851.2), rat (NM_001034912.1), The similarity of the sequence of Xenopus (NM_001126975.1) was 87.80 and 87.449.The similarity of the sequence was 68.471and 67.01and 67.29.The similarity of the sequence was 87.80 and 87.449.The phylogenetic tree was used to analyze the sequence of Xenopus laevis (Xenopus laevis). The results showed that FAM134B gene was highly conserved in the evolution of different species. By comparative analysis, it was found that the CDS region of Luchuan pig FAM134B gene was 129bp more than the Jinhua FAM134B sequence (JX854456.1) published by NCBI, and 371bp, less than the predicted FAM134B sequence (XM_003483804.2). This suggests that the amplified FAM134B gene of Luchuan pig may be another splice. In this study, the complete CDS sequence of FAM134B gene of Luchuan pig was cloned successfully, which provided the basic theory for the future study of FAM134B gene in fat deposition and fat metabolism of Luchuan pig.
【作者单位】：广西壮族自治区畜牧研究所广西家畜遗传改良重点实验室;
【基金】：国家自然科学基金项目(31360544) 广西自然科学基金项目(2011GXNSFA018109,2013GXNSFBA019117) 广西水产畜牧科技推广应用项目(桂渔牧科201452020)共同资助
【分类号】：S828

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