γ-氨基丁酸对大骨鸡生产性能和下丘脑采食调控相关基因调控研究

发布时间：2018-11-29 08:22

【摘要】：为了研究γ-氨基丁酸(GABA)对大骨鸡生产性能和下丘脑采食相关基因表达的影响,将120只21日龄大骨鸡分成4组,分别在基础日粮中添加GABA 0、5、50和500 mg/kg,每组3个重复,每重复10只,试验为期2周。分析各组鸡生产性能并检测下丘脑NPY、AgRp、GABAA-α1、MC4R和POMC基因的mRNA表达量。结果:与对照组相比,日粮中添加5 mg/kg GABA(低剂量)对增重无显著影响(P0.05),50 mg/kg(中剂量)组极显著提高平均日增重(P0.01),500 mg/kg(高剂量)组极显著降低平均日增重(P0.01)。与对照相比,高剂量组大骨鸡公鸡下丘脑GABAA-α_1 mRNA和MC4R mRNA表达显著提高(P0.05),而POMC mRNA和AgRP mRNA极显著降低(P0.01),NPY mRNA无显著变化(P0.05);高剂量组母鸡GABAA-α_1 mRNA极显著降低(P0.01),NPY mRNA显著降低(P0.05),而MC4R mRNA显著提高(P0.05)。试验提示,日粮中添加GABA,对大骨鸡下丘脑采食调控基因表达均有影响,且存在性别差异。
[Abstract]:In order to study the effect of 纬 -aminobutyric acid (GABA) on growth performance and hypothalamic feeding related gene expression in large bone chickens, 120 21-day-old chickens were divided into 4 groups and supplemented with GABA _ 0 _ (50) and 500 mg/kg, respectively in basal diet. The experiment lasted 2 weeks. The production performance of each group was analyzed and the mRNA expression of MC4R and POMC genes in hypothalamus of NPY,AgRp,GABAA- 伪 1 mc4R and POMC were detected. Results: compared with the control group, the dietary supplementation of 5 mg/kg GABA (low dose) had no significant effect on weight gain (P0.05), and the 50 mg/kg (medium dose) group significantly increased the average daily gain (P0.01). The average daily gain was significantly decreased in the 500 mg/kg (high dose) group (P0.01). Compared with the control group, the expression of GABAA- 伪 _ 1 mRNA and MC4R mRNA in hypothalamus of high-dose group was significantly increased (P0.05), while POMC mRNA and AgRP mRNA were significantly decreased (P0.01), NPY mRNA, P0.05). In high dose group, GABAA- 伪 _ 1 mRNA was significantly decreased (P0.01), NPY mRNA significantly decreased (P0.05), while MC4R mRNA was significantly increased (P0.05). The results showed that diet supplemented with GABA, had an effect on the expression of feed intake regulation genes in hypothalamus of large bone chicken, and there were gender differences.
【作者单位】：锦州医科大学畜牧兽医学院/辽宁省畜产品质量与安全工程重点实验室;
【基金】：国家自然科学基金(31272415)
【分类号】：S831.5

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