抗草丁膦转基因油菜与野芥菜的抗性回交3代子1代和子2代的适合度
发布时间：2018-12-08 20:50
【摘要】：如果抗除草剂转基因油菜的抗性基因渗入到野芥菜中,会给野芥菜的防除带来很大困难。因此在抗除草剂转基因油菜环境释放前对抗性基因向野芥菜的渗入开展深入的研究非常必要。以抗草丁膦转基因油菜与野芥菜的携带抗性基因回交3代子1代和子2代(BC3m F2和BC3p F2及BC3m F3和BC3p F3,m表示以野芥菜为母本的回交后代,p表示以野芥菜为父本的回交后代)为材料,在田间条件下研究了它们在不同密度(低密度为15株/区,高密度为30株/区)及不同种植比例(单种,野芥菜与回交后代以4∶1、3∶2、1∶1混种)时的适合度成分和总适合度。结果表明,无论是低密度还是高密度条件下,单种时BC3F2和BC3F3的总适合度均与野芥菜无显著差异。低密度混种时,在4∶1和3∶2下,只有BC3m F3的总适合度与野芥菜无显著差异,其余各后代的总适合度均显著小于野芥菜;以1∶1混种时,只有BC3m F2和BC3m F3的总适合度与野芥菜无显著差异。高密度混种时,3个比例混种下4种供试回交后代的总适合度均显著小于野芥菜。相关性分析结果表明,BC3m F3的各适合度成分都与混种比例不相关。表明携带抗性基因的BC3F2和BC3F3在野外都具有生存定植的可能性,且BC3m F3定植的可能性较其他供试回交后代更大。因此在防范转基因油菜基因逃逸的策略上,在防范初始杂交发生的同时,也应该防范回交后代的产生。
[Abstract]:If herbicide resistant genes of transgenic rapeseed infiltrate into wild mustard, it will bring great difficulty to the control of wild mustard. Therefore, it is necessary to study the penetration of resistance genes into wild mustard before herbicide resistant transgenic rapeseed is released. Transgenic rapeseed resistant to butylphosphine was used to backcross the first and second generations (BC3m F2 and BC3p F2 and BC3m F 3 and BC3p F 3) of the third generation (BC3m F2 and BC3p F2 and BC3m F3 and BC3p F3) to indicate the backcross progenies with wild mustard as the female parent. P the backcross progenies with wild mustard as male parent were used to study their different densities (low density 15 plants / region, high density 30 plants / region) and different planting ratios (single species) under field conditions. The suitable composition and total fitness of wild mustard and backcross progenies were 4: 1: 3: 2: 1: 1). The results showed that there was no significant difference in the total fitness of BC3F2 and BC3F3 between wild mustard and BC3F2 under low or high density. At 4:1 and 3:2, there was no significant difference between the total fitness of BC3m F3 and that of wild mustard, and the total fitness of other progenies was significantly lower than that of wild mustard. When mixed at 1:1, only BC3m F2 and BC3m F 3 had no significant difference between the total fitness of wild mustard and that of BC3m F2 and BC3m F 3. When mixed with high density, the total fitness of four backcross progenies under three proportion hybrids was significantly lower than that of wild mustard. The results of correlation analysis showed that all suitable components of BC3m F _ 3 were not related to the proportion of hybrid species. The results showed that both BC3F2 and BC3F3 carrying resistance genes had the possibility of survival and colonization in the field, and the probability of BC3m F3 colonization was higher than that of other backcross progenies. Therefore, in order to prevent the escape of transgenic rapeseed gene, we should prevent the generation of backcross progeny as well as the initial hybridization.
【作者单位】： 南京农业大学杂草研究室;
【基金】：转基因生物新品种培育科技重大专项子课题(2016ZX08012005-006) 国家自然科学基金项目(31270579)资助
【分类号】：S451;S565.4
本文编号：2368979
[Abstract]:If herbicide resistant genes of transgenic rapeseed infiltrate into wild mustard, it will bring great difficulty to the control of wild mustard. Therefore, it is necessary to study the penetration of resistance genes into wild mustard before herbicide resistant transgenic rapeseed is released. Transgenic rapeseed resistant to butylphosphine was used to backcross the first and second generations (BC3m F2 and BC3p F2 and BC3m F 3 and BC3p F 3) of the third generation (BC3m F2 and BC3p F2 and BC3m F3 and BC3p F3) to indicate the backcross progenies with wild mustard as the female parent. P the backcross progenies with wild mustard as male parent were used to study their different densities (low density 15 plants / region, high density 30 plants / region) and different planting ratios (single species) under field conditions. The suitable composition and total fitness of wild mustard and backcross progenies were 4: 1: 3: 2: 1: 1). The results showed that there was no significant difference in the total fitness of BC3F2 and BC3F3 between wild mustard and BC3F2 under low or high density. At 4:1 and 3:2, there was no significant difference between the total fitness of BC3m F3 and that of wild mustard, and the total fitness of other progenies was significantly lower than that of wild mustard. When mixed at 1:1, only BC3m F2 and BC3m F 3 had no significant difference between the total fitness of wild mustard and that of BC3m F2 and BC3m F 3. When mixed with high density, the total fitness of four backcross progenies under three proportion hybrids was significantly lower than that of wild mustard. The results of correlation analysis showed that all suitable components of BC3m F _ 3 were not related to the proportion of hybrid species. The results showed that both BC3F2 and BC3F3 carrying resistance genes had the possibility of survival and colonization in the field, and the probability of BC3m F3 colonization was higher than that of other backcross progenies. Therefore, in order to prevent the escape of transgenic rapeseed gene, we should prevent the generation of backcross progeny as well as the initial hybridization.
【作者单位】： 南京农业大学杂草研究室;
【基金】：转基因生物新品种培育科技重大专项子课题(2016ZX08012005-006) 国家自然科学基金项目(31270579)资助
【分类号】：S451;S565.4
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