单增李斯特菌srtA基因敲除菌株的构建及其生物学特性

发布时间：2018-12-12 05:16

【摘要】：单核细胞增生性李斯特菌是常见的食源性致病菌,其细胞壁上存在的表面蛋白与其致病性和细菌菌膜的形成密切相关,而srt A基因是介导表面蛋白膜表面定位的关键因子,通过识别特定的蛋白序列将表面蛋白共价结合在细胞壁上,也是单增李斯特菌的重要毒力基因。为深入研究srtA的功能及其对细菌毒力的调控,本研究通过同源重组技术敲除了单增李斯特菌中的srt A基因,并对基因敲除菌株的生物学特性进行了初步研究。通过生长曲线的测定,发现srtA基因敲除株的生长活性低于野生型菌株。进一步利用该菌株对星形胶质细胞系U251的侵袭实验发现,srtA基因敲除菌株的侵袭效率低于野生菌株,提示srtA基因在调控单增李斯特菌的侵袭能力方面发挥重要作用。本研究可为单增李斯特菌毒力基因调控和细菌菌膜的研究提供理论依据。
[Abstract]:Listeria monocytogenes (Listeria monocytogenes) is a common foodborne pathogenic bacteria. The surface proteins on its cell wall are closely related to its pathogenicity and bacterial membrane formation. Srt A gene is the key factor to mediate the surface localization of surface protein membrane. Surface protein covalent binding to the cell wall by recognizing specific protein sequences is also an important virulence gene of Listeria monocytogenes. In order to study the function of srtA and its regulation on bacterial virulence, the srt A gene from Listeria monocytogenes was knocked out by homologous recombination technique, and the biological characteristics of the knockout strain were studied. The growth activity of srtA knockout strain was lower than that of wild type strain. Further, it was found that the invasive efficiency of srtA knockout strain was lower than that of wild strain, suggesting that srtA gene plays an important role in regulating the invasion ability of Listeria monocytogenes. This study can provide theoretical basis for the study of virulence gene regulation and bacterial membrane of Listeria monocytogenes.
【作者单位】：上海理工大学医疗器械与食品学院;
【基金】：上海理工大学博士启动基金资助项目(1000308001) 上海市高校青年教师资助计划项目(10-15-308-802)
【分类号】：TS201.3

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