PJA1及miR-130a基因在口腔肿瘤组织中的表达及相关性分析

发布时间：2018-12-12 04:38

【摘要】：目的研究PJA1及miR-130a在口腔肿瘤组织中的表达情况,明确对肿瘤细胞迁移增殖的影响,并探究两种基因之间的相互作用。方法收集15组口腔肿瘤及其瘤旁组织,提取组织内RNA,应用qRT-PCR法检测组织中PJA1及miR-130a的表达情况;构建PJA1过表达、miR-130a过表达及两者同时过表达的口腔肿瘤细胞株SCC-3,应用MTT实验和Wound scratch assay实验分别检测细胞增殖与迁移的影响。结果 qRT-PCR结果表明PJA1在口腔肿瘤组织中的表达明显低于瘤旁组织(P0.05),miR-130a在口腔肿瘤组织中的表达明显高于瘤旁组织(P0.05);MTT和Wound scratch assay实验结果表明PJA1过表达可以抑制肿瘤细胞的增殖与迁移,miR-130a过表达可以促进肿瘤细胞的增殖与迁移,两者同时过表达PJA1可以抑制miR-130a促进肿瘤细胞增殖与迁移的能力。结论 PJA1在口腔肿瘤组织中低表达,具有抑制肿瘤细胞增殖和迁移的能力;miR-130a在口腔肿瘤组织中高表达,具有促进肿瘤细胞增殖和迁移的能力;miR-130a可能通过抑制了PJA1从而促进肿瘤细胞的增殖和迁移。
[Abstract]:Objective to study the expression of PJA1 and miR-130a in oral tumor tissues, to investigate the effects of PJA1 and miR-130a on the migration and proliferation of tumor cells and to explore the interaction between the two genes. Methods the expressions of PJA1 and miR-130a in 15 groups of oral tumors and their adjacent tissues were detected by qRT-PCR method and RNA, was extracted from the tissues. Overexpression of PJA1, overexpression of miR-130a and overexpression of both in oral tumor cell line SCC-3, were used to detect the effect of cell proliferation and migration by MTT assay and Wound scratch assay, respectively. Results qRT-PCR results showed that the expression of PJA1 in oral tumor tissue was significantly lower than that in adjacent tumor tissue (P0.05), and the expression of miR-130a in oral tumor tissue was significantly higher than that in paratumor tissue (P0.05). The results of MTT and Wound scratch assay experiments showed that the overexpression of PJA1 could inhibit the proliferation and migration of tumor cells, and the overexpression of miR-130a could promote the proliferation and migration of tumor cells. Both overexpression of PJA1 could inhibit the ability of miR-130a to promote the proliferation and migration of tumor cells. Conclusion PJA1 can inhibit the proliferation and migration of tumor cells in oral tumor tissues, and the high expression of miR-130a in oral tumor tissues can promote the proliferation and migration of tumor cells. MiR-130a may promote the proliferation and migration of tumor cells by inhibiting PJA1.
【作者单位】：安徽医科大学附属口腔医院口腔颌面外科;
【基金】：国家自然科学基金(编号:31501103) 安徽省自然科学基金(编号:1508085QC62)
【分类号】：R739.8

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