黑杨应答杨生褐盘二孢专化型侵染的基因差异表达

发布时间：2018-12-12 16:24

【摘要】：[目的]杨生褐盘二孢的单芽管专化型和多芽管专化型分别引起白杨组和黑杨组(包括青杨组)杨树的褐斑病,其分化机制有待阐明。[方法]本文在专化性互作体系建立的基础上,利用荧光染色标记研究了病菌孢子在专化性寄主侵染过程中的萌发发育情况,并通过RT-qPCR分析了黑杨寄主抗病相关基因在应答两专化型侵染过程中的不同表达。[结果]结果发现多芽管分生孢子在黑杨寄主上能完成发育并成功侵染,单芽管分生孢子也能成功萌发和发育,但不能侵入黑杨寄主;黑杨抗性基因在两专化型侵染发育过程中均能被诱导表达,但在表达时间和表达量上,除基因WRKY89的表达较为一致外,病程相关基因PR5、PR10、NPR1和LAR3在二者间的表达存在差异。[结论]研究结果初步表明病原专化型可能是寄主与病原专性互作的表型,这一认识将有助于进一步探明杨树褐斑病病原专化型形成和分化的机制。
[Abstract]:[objective] to induce brown spot of poplar in poplar group and black poplar group (including poplar group) by single bud tube specialization type and multi bud tube specialization type, and its differentiation mechanism needs to be elucidated. [methods] based on the establishment of specific interaction system, the germination and development of spores in the process of infection by specific host were studied by fluorescent staining. RT-qPCR was used to analyze the different expression of resistance related genes in two specific types of infection of Populus nigra. [results] it was found that the multi-bud conidia could be successfully developed and infected on the host of Populus nigra, and that the conidia of single bud tube could also germinate and develop successfully, but could not invade the host of Populus nigra. The resistant genes of Populus nigra could be induced to express in the course of infection and development of two specific types, but the expression time and quantity of WRKY89 were consistent, the expression of pathogenesis-related genes PR5,PR10,NPR1 and LAR3 were different between them. [conclusion] the results showed that the pathogen-specific type might be the phenotype of the interaction between host and pathogen specificity, and this understanding will be helpful to further study the mechanism of pathogen specific type formation and differentiation of poplar brown spot disease.
【作者单位】：中国林业科学研究院森林生态环境与保护研究所;北京林业大学林学院;南京林业大学南方现代林业协同创新中心;
【基金】：基金项目:效应物在杨盘二孢半活养方式及亲和寄主致病性中的作用(31370645) 水解纤维素产烃树木内生菌资源利用技术引进(2013-4-10) 芯片通量病原检测技术在杨树病害防控中的应用([2016]10号)
【分类号】：S763.7

【相似文献】