靶向家蚕丝素重链基因的锌指蛋白结构域的人工构建
发布时间：2018-12-12 23:52
【摘要】：将多个可特异识别并结合DNA的锌指单体串联在一起即可构成锌指蛋白结构域,若将其与不同的功能域因子融合,则会构建出有很多用途的人工蛋白分子,从而实现对基因组的各种定点修饰或调控。以家蚕丝素重链基因为研究对象,首先利用Zinc Finger Tools软件筛选到了一个合适的靶标位点,再采用模块组装技术,通过酶切、片段回收、连接、转化等步骤成功将多个锌指单体串联在一起,构建出一对靶向家蚕丝素重链基因特定位点的由4个锌指单体组成的锌指蛋白结构域,这为将来定点修饰或改造家蚕丝素基因,进一步提高蚕丝质量和产量,以及大幅度提升家蚕生物反应器对外源蛋白的表达能力打下了良好的基础。
[Abstract]:Several zinc finger monomers that can specifically recognize and bind to DNA can be linked together to form zinc finger protein domains. If they are fused with different functional domain factors, artificial protein molecules with many uses will be constructed. In order to achieve a variety of genomes fixed-point modification or regulation. The silkworm fibroin heavy chain gene was used as the research object. A suitable target site was screened by using Zinc Finger Tools software, and then the module assembly technique was adopted, which was digested by enzyme, recovered and connected. Several zinc finger monomers were successfully linked together to construct a pair of zinc finger protein domains composed of 4 zinc finger monomers at specific sites of silkworm silk heavy chain gene. The further improvement of silk quality and yield, as well as the improvement of silkworm bioreactor to foreign protein expression ability laid a good foundation.
【作者单位】： 中山大学有害生物控制与资源利用国家重点实验室//昆虫学研究所;
【基金】：广东省科技计划项目(2014A010107009)
【分类号】：Q78
[Abstract]:Several zinc finger monomers that can specifically recognize and bind to DNA can be linked together to form zinc finger protein domains. If they are fused with different functional domain factors, artificial protein molecules with many uses will be constructed. In order to achieve a variety of genomes fixed-point modification or regulation. The silkworm fibroin heavy chain gene was used as the research object. A suitable target site was screened by using Zinc Finger Tools software, and then the module assembly technique was adopted, which was digested by enzyme, recovered and connected. Several zinc finger monomers were successfully linked together to construct a pair of zinc finger protein domains composed of 4 zinc finger monomers at specific sites of silkworm silk heavy chain gene. The further improvement of silk quality and yield, as well as the improvement of silkworm bioreactor to foreign protein expression ability laid a good foundation.
【作者单位】： 中山大学有害生物控制与资源利用国家重点实验室//昆虫学研究所;
【基金】：广东省科技计划项目(2014A010107009)
【分类号】：Q78
【相似文献】
相关期刊论文 前10条
1 田路明;黄丛林;张秀海;张潞生;吴忠义;;逆境相关植物锌指蛋白的研究进展[J];生物技术通报;2005年06期
2 林燕;周小秋;;锌与锌指蛋白对机体贫血的影响[J];动物营养学报;2006年02期
3 赵楠;赵飞;李玉花;;锌指蛋白结构及功能研究进展[J];生物技术通讯;2009年01期
4 洪洋;宋冰;王丕武;王贺;;锌指蛋白结构及其功能研究[J];才智;2010年35期
5 刘卓;张飞;赵心清;白凤武;;锌指蛋白及人工锌指蛋白对微生物代谢影响的研究进展[J];生物工程学报;2014年03期
6 王东,杨金水;棉花类耐盐锌指蛋白基因的克隆与结构分析[J];复旦学报(自然科学版);2002年01期
7 李晓波;张俊武;;真核生物中锌指蛋白的结构与功能[J];中国生物化学与分子生物学报;2009年03期
8 鹿培源,贾弘，
本文编号：2375472
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2375472.html
最近更新
教材专著
