巴西橡胶树生长素响应HbJAR1基因克隆与表达分析

发布时间：2018-12-12 23:56

【摘要】：JAR1基因是植物生长素响应基因,具有维持植物体内生长素浓度动态平衡和增强植物抗逆性的功能。为了阐明巴西橡胶树中JAR1基因的结构与功能,本研究利用Q-PCR技术在橡胶树CATAS7-33-97叶片中扩增了JAR1基因,其推导氨基酸含有JAR1蛋白特征性的GH3 superfamily结构域,无信号肽,无跨膜区域,定位在细胞质中,命名为HbJAR1。表达分析结果表明:该基因在橡胶树叶片中的表达量受光照强度、机械伤害和白粉菌侵染显著上调,吲哚乙酸(IAA)、茉莉酸(JA)、乙烯利(ET)、脱落酸(ABA)和赤霉素(GA3)对HbJAR1在橡胶树中的表达也起到不同程度的上调作用。表明HbJAR1基因属于植物JAR1家族,与橡胶树对光照、伤害等逆境响应有关。
[Abstract]:JAR1 gene is a plant auxin response gene, which has the function of maintaining dynamic balance of auxin concentration in plants and enhancing plant resistance to stress. In order to elucidate the structure and function of JAR1 gene in Hevea brasiliensis, the JAR1 gene was amplified by Q-PCR technique in CATAS7-33-97 leaves of Hevea brasiliensis. The deduced amino acids contained GH3 superfamily domain with characteristic JAR1 protein and no signal peptide. No transmembrane region, located in cytoplasm, named HbJAR1. The results of expression analysis showed that the expression of the gene was significantly up-regulated by light intensity, mechanical injury and powdery mildew infection, and (JA), ethephon (ET), (JA),) in indole acetic acid (IAA), jasmonate was significantly up-regulated by light intensity, mechanical injury and powdery mildew infection. Abscisic acid (ABA) and gibberellin (GA3) also up-regulated the expression of HbJAR1 in Hevea. The results showed that HbJAR1 gene belonged to the JAR1 family of plants, and was related to the response of Hevea to light, injury and other stresses.
【作者单位】：中国热带农业科学院科技信息研究所;海南大学材料与化工学院;三亚市现代农业检验检测预警防控中心;海南省热带生物资源可持续利用重点实验室海南大学热带农林学院;
【基金】：国家自然科学基金(No.31660204、31660187) 中国热带农业科学院基本科研业务费专项资金(No.1630072016005)
【分类号】：Q943.2;S794.1

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