毛果杨基因PtNRT2.7的功能初步鉴定与分析

发布时间：2018-12-13 00:39

【摘要】：本研究从毛果杨基因组中克隆一个NRT2家族的新基因Pt NRT2.7。生物信息学分析显示,其编码的蛋白不存在信号肽及切割位点,属于非分泌蛋白;具有11个跨膜结构域,亲水区与输水区交替分布,主要分布于细胞膜,而且该基因的亚细胞定位分析显示该蛋白定位于细胞膜,综合以上结果可判断此蛋白为膜蛋白。对其在毛果杨的表达特征分析显示,Pt NRT2.7基因主要在叶片中表达,其中成熟叶中的表达量最高;Pt NRT2.7基因受硝酸盐诱导并在1 h时达到表达峰值;SA、JA、GA或IAA激素处理可诱导Pt NRT2.7基因表达,而在Eth、ABA或Na Cl处理下该基因的表达受抑制。利用农杆菌花序侵染法转化野生型拟南芥和突变体(nrt2.7),显示在KNO3浓度大于0.1mmol/L时,Pt NRT2.7过表达株系相比较拟南芥野生型和突变体株系促进了根的生长,而atnrt2.7突变体在KNO3浓度大于1 mmol/L的条件下抑制了根的生长,且Pt NRT2.7过表达atnrt2.7突变体株系可恢复正常生长。研究结果表明,Pt NRT2.7可以由硝酸盐和激素诱导并增强植物根系的生长,提高氮素的吸收利用。
[Abstract]:In this study, a novel gene Pt NRT2.7. of NRT2 family was cloned from the genome of Populus tomentosa. Bioinformatics analysis showed that the encoded protein did not contain signal peptide and cleavage site, so it belonged to non-secretory protein. There were 11 transmembrane domains, the hydrophilic region and the water transport region were distributed alternately, mainly distributed in the cell membrane, and the subcellular localization analysis of the gene showed that the protein was located on the cell membrane. The expression characteristics of Pt NRT2.7 gene in poplar were analyzed. The results showed that the Pt NRT2.7 gene was mainly expressed in the leaves, and the Pt NRT2.7 gene was induced by nitrate and reached the peak at 1 h. SA,JA,GA or IAA hormone treatment could induce the expression of Pt NRT2.7 gene, but under Eth,ABA or Na Cl treatment, the expression of Pt NRT2.7 gene was inhibited. Wild type Arabidopsis thaliana and mutant (nrt2.7) were transformed by Agrobacterium tumefaciens inflorescence. When KNO3 concentration was higher than 0.1mmol/L, Pt NRT2.7 overexpression lines promoted root growth compared with Arabidopsis wild-type and mutant lines. The atnrt2.7 mutant inhibited root growth when the concentration of KNO3 was more than 1 mmol/L, and the Pt NRT2.7 overexpression atnrt2.7 mutant could return to normal growth. The results showed that Pt NRT2.7 could be induced by nitrate and hormone to enhance the growth of plant roots and increase the uptake and utilization of nitrogen.
【作者单位】：林木育种国家工程实验室北京林业大学生物科学与技术学院;
【基金】：林业公益性行业科研专项(201304301) 国家自然科学基金项目(31270656) 高等学校学科创新引智计划项目(111 Project;B13007)
【分类号】：S792.11

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