佛手果形发育观察及果形相关基因表达分析

发布时间：2018-12-13 21:17

【摘要】：通过超景深显微镜和扫描电镜观察不同发育阶段的佛手(Citrus medica L.var.sarcodactylis Swingle)花芽形态,发现佛手指状部分并非发育自柱头,而是来自雌蕊子房。同时,在佛手中分离了CmsSUN20、CmsOFP7、CmsOFP12和CmsYABBY5等4个果形相关基因的全长,与甜橙序列比对的相似性大于95%,构建进化树分析发现其与甜橙聚在一个分支。收集佛手和香橼(C.medica L.)果实发育相同阶段不同组织(叶片,花瓣,雌蕊,雄蕊)材料,以qRT-PCR法分析了上述4个基因的表达。结果表明CmsSUN20在佛手雌蕊的表达显著高于香橼,在其他组织中差异不显著或相反,表明该基因可能与佛手雌蕊发育有关。CmsYABBY5在佛手雌蕊和雄蕊中都显著高表达,而在叶片中却显著低表达,表明CmsYABBY5有可能对佛手雌蕊和雄蕊发育有重要作用。而CmsOFP7和CmsOFP12在佛手叶片、雌蕊和雄蕊中均高表达,由于并非特异在雌蕊中高表达,所以可能并不对佛手果形建成起决定作用。
[Abstract]:The floral bud morphology of (Citrus medica L.var.sarcodactylis Swingle in different stages of development was observed by means of hyper-depth microscope and scanning electron microscope. It was found that the finger part of the Buddha developed not from the stigma but from the pistil ovary. At the same time, four fruit shape related genes, CmsSUN20,CmsOFP7,CmsOFP12 and CmsYABBY5, were isolated from the phalanx, and the similarity of alignment with orange sequence was greater than 95. The phylogenetic tree analysis showed that it was clustered in a branch with sweet orange. C.medica L. The expression of the four genes in different tissues (leaves, petals, pistil, stamens) at the same stage of fruit development was analyzed by qRT-PCR method. The results showed that the expression of CmsSUN20 in pistil was significantly higher than that in Citron, but there was no significant difference in other tissues, suggesting that the expression of CmsSUN20 might be related to the development of pistil. CmsYABBY5 was significantly overexpressed in both pistil and stamen of Vertex. However, the expression of CmsYABBY5 was significantly lower in leaves, suggesting that CmsYABBY5 may play an important role in the development of pistil and stamen. However, CmsOFP7 and CmsOFP12 were highly expressed in the leaves, pistil and stamen, but they were not specifically expressed in the pistil, so they may not play a decisive role in the fruit formation.
【作者单位】：浙江师范大学化学与生命科学学院;
【基金】：浙江省科技计划面上项目(2008C22002) 国家留学基金委地方合作项目(201308330248)
【分类号】：S666.9

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