Sema4D基因沉默对骨髓间充质干细胞成骨及成脂肪分化相关基因表达的影响
发布时间：2018-12-23 18:28
【摘要】：为了探讨Sema4D基因对绝经后骨质疏松患者骨髓间充质干细胞(BMMSCs)成骨及成脂肪分化能力的影响,设计3条Sema4D基因的siRNAs,分别通过显微观察和蛋白质免疫印迹(Western blot)鉴定其对BMMSCs生长形态以及对Sema4D蛋白表达的影响.进一步利用实时荧光定量PCR(real-time PCR)和Western blot检测Sema4D基因沉默对成骨指标抗碱性磷酸酶(ALP)、骨钙素(BGP)、胶原蛋白Ⅰ(CollagenⅠ)和成脂肪分化指标脂蛋白酶(LPL)、脂肪细胞结合蛋白2(AP-2)、瘦素(Leptin)表达的影响以及对骨保护素和可溶性细胞核因子-κB受体活化因子及配基(OPG/RANKL/RANK)系统的影响.结果显示Sema4D基因沉默后,绝经后骨质疏松患者BMMSCs中ALP、BGP、CollagenⅠ的表达水平明显升高,LPL、AP-2、Leptin的表达水平也明显升高;OPG的表达水平明显升高,而RANKL和RANK的表达水平明显下降.由上述结果可知,Sema4D基因可以抑制BMMSCs成骨分化和成脂肪分化相关基因的表达,可能通过下调OPG/RANKL/RANK系统相关基因的表达来实现.
[Abstract]:To investigate the effect of Sema4D gene on the osteogenesis and adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) in postmenopausal patients with osteoporosis, three siRNAs, fragments of Sema4D gene were designed. The morphology of BMMSCs and the expression of Sema4D protein were identified by microscopical observation and Western blot (Western blot). Furthermore, real-time fluorescence quantitative PCR (real-time PCR) and Western blot were used to detect Sema4D gene silencing and osteogenic index (ALP), osteocalcin (BGP), collagen 鈪，
本文编号：2390085
[Abstract]:To investigate the effect of Sema4D gene on the osteogenesis and adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) in postmenopausal patients with osteoporosis, three siRNAs, fragments of Sema4D gene were designed. The morphology of BMMSCs and the expression of Sema4D protein were identified by microscopical observation and Western blot (Western blot). Furthermore, real-time fluorescence quantitative PCR (real-time PCR) and Western blot were used to detect Sema4D gene silencing and osteogenic index (ALP), osteocalcin (BGP), collagen 鈪，
本文编号：2390085
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