乙酰辅酶A羧化酶B基因多态性与2型糖尿病的相关性研究

发布时间：2018-12-25 17:48

【摘要】：目的探讨乙酰辅酶A羧化酶B(ACACB)基因(rs2268388及rs2268393)多态性与中国昆明地区汉族人群T2DM的相关性。方法分别运用聚合酶链反应-限制性片段长度多态性(PCRRFLP)技术及Taqman探针技术,在昆明地区汉族人群中对184例T2DM患者和70名健康对照者(NC)的ACACB基因(rs2268388及rs2268393)多态性进行检测,并比较分析各组间基因型、等位基因频率、相关临床和生化指标。结果 T2DM组和NC组ACACB基因rs2268393各基因型和等位基因频率比较,差异无统计学意义(χ2=4.810,P=0.09;χ2=1.29,P=0.24);T2DM组ACACB基因rs2268388AA基因型和A等位基因频率高于NC组(χ2=10.469,P=0.005;χ2=4.71,P=0.007);T2DM组ACACB基因rs2268388及rs2268393位点AABB基因型分布频率高于NC组(χ2=10.526,P=0.001);AABB基因型是T2DM发生的危险因素(OR:1.44,95%CI:1.32~1.57);二分类Logistic回归分析表明,ACACB基因rs2268388AA基因型、高BMI及高WHR是T2DM发生的危险因素,HDL-C是T2DM发生的保护因素。结论在昆明地区汉族人群中,ACACB基因rs2268388AA基因型可能与T2DM发生相关。
[Abstract]:Objective to investigate the association between the polymorphism of acetyl coA carboxylase B (ACACB) gene (rs2268388 and rs2268393) and T2DM in Kunming Han population. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) and Taqman probe technique were used, respectively. The polymorphisms of ACACB gene (rs2268388 and rs2268393) of (NC) were detected in 184 T2DM patients and 70 healthy controls in Kunming Han population. The genotype, allele frequency, clinical and biochemical indexes were compared and analyzed. Results there was no significant difference in the frequency of rs2268393 genotypes and alleles of ACACB gene between T2DM group and NC group (蠂 ~ (2) 4.810) P ~ (0.09), 蠂 ~ (2 +) ~ (1.29) (P ~ (0.24). The frequency of rs2268388AA genotype and A allele of ACACB gene in T2DM group was higher than that in NC group (蠂 2 + 10.469), and the frequency of ACACB rs2268388 and rs2268393 AABB genotype in T2DM group was higher than that in NC group (蠂 2 + 10.469) (蠂 2 + 10. 526 P + 0. 001), and the frequency of ACACB gene rs2268388 and rs2268393 allele in T2DM group was higher than that in NC group (蠂 2 + 10. 526 P + 0. 001). AABB genotype was the risk factor of T2DM (OR:1.44,95%CI:1.32~1.57), and ACACB gene rs2268388AA genotype, high BMI and high WHR were risk factors of T2DM, HDL-C was the protective factor of T2DM. Conclusion the rs2268388AA genotype of ACACB gene may be associated with T2DM in Kunming Han population.
【作者单位】：昆明医科大学第一附属医院糖尿病科;昆明医科大学第一附属医院检验科;
【基金】：国家自然科学基金(30760087、81160104) 云南省自然科学基金面上项目(2004C0066M)
【分类号】：R587.1

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