异源表达胡杨PeCPD基因提高烟草对盐、高氮和干旱胁迫的抗性
发布时间：2018-12-27 15:02
【摘要】：油菜素类固醇(Brassinosteroids,BRs)在调节植物生长发育和提高植物对非生物胁迫的抗性中起着重要作用,CPD(Constitutive Photomorphogenesis and Dwarfism)基因编码一种甾醇类23a羟化酶,在BR的生物合成中发挥着重要作用.本研究克隆胡杨Pe CPD基因的c DNA序列,并利用农杆菌介导法,将Pe CPD基因植物过表达载体p BI121-35S::Pe CPD转化野生型烟草,筛选得到Pe CPD基因过表达的转基因烟草植株,并利用Na Cl、高氮和甘露醇分别对转基因和野生型烟草进行胁迫处理,检测转基因烟草的耐受性和相关的生理生化指标.结果表明:(1)在正常条件下,转基因烟草的鲜重是野生型烟草的1.47倍;(2)在Na Cl、高氮以及甘露醇的胁迫处理下,转基因烟草植株的根长、叶片叶绿素和类胡萝卜素的含量均显著高于野生型植株,抗氧化酶SOD和POD活性以及渗透调节物质脯氨酸、可溶性蛋白和可溶性糖的含量也同样表现为转基因烟草显著高于野生型,而野生型植株的丙二醛(MDA)含量显著高于转基因植株.因此在烟草中异源过表达胡杨Pe CPD基因不但能够增加烟草的生物量,而且能够提高烟草对盐、高氮和干旱胁迫的抵抗能力.
[Abstract]:Rapesin steroids (Brassinosteroids,BRs) play an important role in regulating plant growth and development and enhancing plant resistance to abiotic stress. The, CPD (Constitutive Photomorphogenesis and Dwarfism) gene encodes a sterol 23a hydroxylase. It plays an important role in the biosynthesis of BR. In this study, the c DNA sequence of Pe CPD gene of Populus euphratica was cloned and transformed into wild type tobacco by means of Agrobacterium tumefaciens mediated by Pe CPD gene plant overexpression vector p BI121-35S::Pe CPD. Transgenic tobacco plants with Pe CPD gene overexpression were screened. Na Cl, high nitrogen and mannitol were used to stress transgenic tobacco and wild type tobacco respectively to detect tolerance and related physiological and biochemical indexes of transgenic tobacco. The results showed that: (1) under normal conditions, the fresh weight of transgenic tobacco was 1.47 times of that of wild tobacco; (2) under the stress of Na Cl, high nitrogen and mannitol, the root length, chlorophyll and carotenoid content of transgenic tobacco plants were significantly higher than those of wild-type plants. The activities of antioxidant enzymes SOD and POD and the contents of proline, soluble protein and soluble sugar also showed that transgenic tobacco was significantly higher than wild-type tobacco. The malondialdehyde (MDA) content of wild type plants was significantly higher than that of transgenic plants. Therefore, heterologous expression of Populus euphratica Pe CPD gene in tobacco could not only increase the biomass of tobacco, but also enhance the resistance of tobacco to salt, high nitrogen and drought stress.
【作者单位】： 西南科技大学生命科学与工程学院;
【基金】：国家自然科学基金项目(31270660) 四川省青年科技基金项目(2014JQ0016) 西南科技大学博士启动资金和西南科技大学杰出青年基金项目资助~~
【分类号】：Q943.2
本文编号：2393249
[Abstract]:Rapesin steroids (Brassinosteroids,BRs) play an important role in regulating plant growth and development and enhancing plant resistance to abiotic stress. The, CPD (Constitutive Photomorphogenesis and Dwarfism) gene encodes a sterol 23a hydroxylase. It plays an important role in the biosynthesis of BR. In this study, the c DNA sequence of Pe CPD gene of Populus euphratica was cloned and transformed into wild type tobacco by means of Agrobacterium tumefaciens mediated by Pe CPD gene plant overexpression vector p BI121-35S::Pe CPD. Transgenic tobacco plants with Pe CPD gene overexpression were screened. Na Cl, high nitrogen and mannitol were used to stress transgenic tobacco and wild type tobacco respectively to detect tolerance and related physiological and biochemical indexes of transgenic tobacco. The results showed that: (1) under normal conditions, the fresh weight of transgenic tobacco was 1.47 times of that of wild tobacco; (2) under the stress of Na Cl, high nitrogen and mannitol, the root length, chlorophyll and carotenoid content of transgenic tobacco plants were significantly higher than those of wild-type plants. The activities of antioxidant enzymes SOD and POD and the contents of proline, soluble protein and soluble sugar also showed that transgenic tobacco was significantly higher than wild-type tobacco. The malondialdehyde (MDA) content of wild type plants was significantly higher than that of transgenic plants. Therefore, heterologous expression of Populus euphratica Pe CPD gene in tobacco could not only increase the biomass of tobacco, but also enhance the resistance of tobacco to salt, high nitrogen and drought stress.
【作者单位】： 西南科技大学生命科学与工程学院;
【基金】：国家自然科学基金项目(31270660) 四川省青年科技基金项目(2014JQ0016) 西南科技大学博士启动资金和西南科技大学杰出青年基金项目资助~~
【分类号】：Q943.2
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