质体基因工程中选择标记基因研究进展
发布时间：2018-12-28 08:50
【摘要】：与细胞核基因工程相比,质体基因工程能更安全、精确和高效地对外源基因进行表达,作为下一代转基因技术已广泛用于基础研究和生物技术应用领域。与细胞核基因工程一样,质体基因工程中也需要合适的选择标记基因用于转化子的筛选和同质化,但基于质体基因组的多拷贝性和母系遗传特点,转化子的同质化需要一个长期的筛选过程,这就决定了质体基因工程中选择标记基因的选择标准将不同于细胞核基因工程中广泛使用的现行标准。目前,质体基因工程的遗传转化操作中使用较多的是抗生素选择标记基因,出于安全性考虑,需要找到可替换、安全的选择标记基因或有效的标记基因删除方法。本文在对质体基因工程研究的相关文献分析基础之上,对主要使用的选择标记基因及其删除体系进行了综述,并对比了其优缺点,同时探讨了质体基因工程中所使用的报告基因,以期为现有选择标记基因及其删除体系的改进和开发提供一定参考,进一步推动质体基因工程,尤其是单子叶植物质体基因工程的发展。
[Abstract]:Compared with nuclear genetic engineering, plastid gene engineering can express foreign genes more safely, accurately and efficiently. As the next generation of transgenic technology, plastids have been widely used in basic research and biotechnology applications. Like nuclear genetic engineering, plastids engineering requires suitable selection of marker genes for screening and homogenization of transformants, but based on multiple copies of the plastids genome and maternal heredity, The homogenization of transformants requires a long-term screening process, which determines that the selection criteria for marker genes in plastids genetic engineering will be different from the existing criteria widely used in nuclear genetic engineering. At present, antibiotic selective marker genes are used in the genetic transformation of plastids. For safety reasons, it is necessary to find alternative and safe selective marker genes or effective marker gene deletion methods. In this paper, based on the literature analysis of plastid gene engineering, the selective marker genes and their deletion systems are reviewed, and their advantages and disadvantages are compared. At the same time, the report genes used in plastid gene engineering are discussed in order to provide some reference for the improvement and development of the existing selective marker gene and its deletion system, and to further promote the plastid gene engineering. In particular, the development of genetic engineering of monocotyledonous plastids.
【作者单位】： 长江大学主要粮食作物产业化湖北省协同创新中心;长江大学生命科学学院;长江大学农学院;
【基金】：主要粮食作物产业化湖北省协同创新中心开放基金项目(编号:2015MS006)资助~~
【分类号】：Q943.2
本文编号：2393752
[Abstract]:Compared with nuclear genetic engineering, plastid gene engineering can express foreign genes more safely, accurately and efficiently. As the next generation of transgenic technology, plastids have been widely used in basic research and biotechnology applications. Like nuclear genetic engineering, plastids engineering requires suitable selection of marker genes for screening and homogenization of transformants, but based on multiple copies of the plastids genome and maternal heredity, The homogenization of transformants requires a long-term screening process, which determines that the selection criteria for marker genes in plastids genetic engineering will be different from the existing criteria widely used in nuclear genetic engineering. At present, antibiotic selective marker genes are used in the genetic transformation of plastids. For safety reasons, it is necessary to find alternative and safe selective marker genes or effective marker gene deletion methods. In this paper, based on the literature analysis of plastid gene engineering, the selective marker genes and their deletion systems are reviewed, and their advantages and disadvantages are compared. At the same time, the report genes used in plastid gene engineering are discussed in order to provide some reference for the improvement and development of the existing selective marker gene and its deletion system, and to further promote the plastid gene engineering. In particular, the development of genetic engineering of monocotyledonous plastids.
【作者单位】： 长江大学主要粮食作物产业化湖北省协同创新中心;长江大学生命科学学院;长江大学农学院;
【基金】：主要粮食作物产业化湖北省协同创新中心开放基金项目(编号:2015MS006)资助~~
【分类号】：Q943.2
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1 侯丙凯,陈正华;高等植物的质体基因转化[J];生物工程进展;2000年01期
2 金桂英,魏文雄,章宁,刘中柱;满江红细胞质遗传和变异的初步研究[J];植物学报;1996年02期
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