不同砧木嫁接黄瓜蜡质合成基因表达分析及其miRNA预测

发布时间：2018-12-28 10:28

【摘要】：为研究不同砧木嫁接中黄瓜果实蜡质合成相关基因的表达量变化,本试验以3461黄瓜为接穗,黑籽南瓜和白籽南瓜为砧木,研究黑籽南瓜嫁接株、白籽南瓜嫁接株和自根苗不同果实生长时期(开花前2 d、开花当天、开花后4 d、开花后6 d、开花后8 d)中蜡质合成基因(Cs CER1、Cs CER3、Cs CER4、Cs CER6、Cs CER8、Cs CER10)的表达变化,并利用RNAhybrid、psRNATarget及RegRNA软件对调控这6个蜡质基因的microRNA(miRNA)作出预测。结果表明,不同砧木嫁接处理中蜡质基因表达差异主要体现在开花后4 d的果实中,Cs CER1、Cs CER3、Cs CER6、Cs CER8、Cs CER10基因在黑籽南瓜嫁接株中的表达量明显高于白籽南瓜嫁接株和自根苗。miRNA预测结果显示,cme-miR164可能调控Cs CER1、Cs CER3、Cs CER4、Cs CER6和Cs CER10基因,cme-miR168可能调控Cs CER3、Cs CER4、Cs CER6和Cs CER10基因,cme-miR390可能调控Cs CER6、Cs CER8和Cs CER10基因。推测Cs CER1、Cs CER3、Cs CER6、Cs CER8、Cs CER10这5个基因与黑籽南瓜嫁接导致的蜡质含量增加密切相关,cme-miR164、cme-miR168和cme-miR390可能参与调控黄瓜蜡质合成。本研究结果为探究嫁接导致蜡质含量变化的机制提供了新的理论依据。
[Abstract]:In order to study the expression changes of the genes related to wax synthesis in cucumber fruit grafted with different rootstocks, 3461 cucumber was used as scion and black seed pumpkin and white seed pumpkin as rootstock to study the grafted plant of black seed pumpkin. Waxy Synthetical genes (Cs CER1,Cs CER3,Cs CER4,Cs CER6,Cs CER8,) in White seed Pumpkin grafted plants and Self-rooted Seedling at different growth stages (2 days before flowering, 4 days after flowering, 6 days after flowering and 8 days after flowering) RNAhybrid,psRNATarget and RegRNA software were used to predict the expression of microRNA (miRNA) in these six waxy genes. The results showed that the difference of waxy gene expression in different rootstock grafting treatments was mainly reflected in the fruit of 4 days after anthesis, Cs CER1,Cs CER3,Cs CER6,Cs CER8,. The expression of Cs CER10 gene in grafted plants of black seed pumpkin was significantly higher than that in grafted plants and self-rooted seedlings of white seed pumpkin. MiRNA predicted that cme-miR164 might regulate Cs CER1,Cs CER3,Cs CER4,Cs CER6 and Cs CER10 genes, and cme-miR168 might regulate Cs CER3,. Cs CER4,Cs CER6 and Cs CER10 genes, cme-miR390 may regulate Cs CER6,Cs CER8 and Cs CER10 genes. It is inferred that the five genes of Cs CER1,Cs CER3,Cs CER6,Cs CER8,Cs CER10 are closely related to the increase of wax content caused by grafted black seed pumpkin. Cme-miR164,cme-miR168 and cme-miR390 may be involved in regulating the waxy synthesis of cucumber. The results of this study provide a new theoretical basis for exploring the mechanism of wax content change caused by grafting.
【作者单位】：山西农业大学园艺学院;
【基金】：山西省农业科技攻关项目(20150311010-2) 山西省煤基重大科技攻关资助项目(FT201402) 山西农业大学博士科研启动项目(2015YJ14)
【分类号】：S642.2

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