玉米耐盐基因ZmHKT1;5在烟草中的功能验证
发布时间：2018-12-30 17:47
【摘要】：HKT类基因是与植物耐盐性密切相关的一类基因。在作物中HKT蛋白可通过排出Na+来维持植物体内的Na~+/K~+平衡,从而影响植物耐盐性。通过在烟草中过表达玉米ZmHKT1;5基因,验证该基因具有提高植物耐盐性的作用。结果表明,过表达ZmHKT1;5基因的T0代材料即显示出叶片耐盐能力的明显提高;T2代转基因株系种子在含盐培养基上的发芽能力明显强于野生型材料,T2代转基因株系幼苗阶段的耐盐能力也得到了明显的提高。通过比较在盐胁迫后2月龄的转基因材料和野生型材料的生理指标,发现野生型材料中MDA和H_2O_2的含量相较转基因材料发生了更为明显的上升,说明转基因材料中过表达ZmHKT1;5基因有效降低了盐胁迫引起的过氧化物积累。综合转基因验证的结果,证明ZmHKT1;5基因具有提高植物耐盐性的作用。
[Abstract]:HKT genes are closely related to salt tolerance in plants. HKT protein can maintain the Na~ / K ~ balance in plants by excreting Na, thus affecting the salt tolerance of plants. By overexpressing maize ZmHKT1;5 gene in tobacco, it was proved that this gene could improve plant salt tolerance. The results showed that the T0 generation material which over-expressed ZmHKT1;5 gene showed that the salt-tolerant ability of leaves was obviously improved. The germinating ability of T2 transgenic lines on salt-containing medium was obviously stronger than that of wild-type materials, and the salt tolerance of T2 transgenic lines was also improved obviously in seedling stage. By comparing physiological indexes of transgenic and wild transgenic materials at 2 months of age after salt stress, it was found that the contents of MDA and H_2O_2 in wild materials were significantly higher than those in transgenic materials. It shows that ZmHKT1; is overexpressed in transgenic materials. 5 gene effectively reduced peroxide accumulation induced by salt stress. The results showed that ZmHKT1;5 gene could improve the salt tolerance of plants.
【作者单位】： 吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室;
【基金】：国家自然科学基金(31100242)
【分类号】：Q943.2;S513
本文编号：2395921
[Abstract]:HKT genes are closely related to salt tolerance in plants. HKT protein can maintain the Na~ / K ~ balance in plants by excreting Na, thus affecting the salt tolerance of plants. By overexpressing maize ZmHKT1;5 gene in tobacco, it was proved that this gene could improve plant salt tolerance. The results showed that the T0 generation material which over-expressed ZmHKT1;5 gene showed that the salt-tolerant ability of leaves was obviously improved. The germinating ability of T2 transgenic lines on salt-containing medium was obviously stronger than that of wild-type materials, and the salt tolerance of T2 transgenic lines was also improved obviously in seedling stage. By comparing physiological indexes of transgenic and wild transgenic materials at 2 months of age after salt stress, it was found that the contents of MDA and H_2O_2 in wild materials were significantly higher than those in transgenic materials. It shows that ZmHKT1; is overexpressed in transgenic materials. 5 gene effectively reduced peroxide accumulation induced by salt stress. The results showed that ZmHKT1;5 gene could improve the salt tolerance of plants.
【作者单位】： 吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室;
【基金】：国家自然科学基金(31100242)
【分类号】：Q943.2;S513
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，本文编号：2395921
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