过表达海马齿SpSOS1基因能增强转基因拟南芥的耐盐性

发布时间：2019-01-02 15:53

【摘要】：细胞膜Na~+/H~+逆转运蛋白SOS1在植物耐盐过程中起重要作用。本研究将盐生植物海马齿中的细胞膜Na~+/H~+逆转运蛋白基因SpSOS1构建到植物双元表达载体pCAMBIA1304上,采用农杆菌介导的方法将SpSOS1基因转入到拟南芥中,在含有50μg/mL潮霉素B的MS培养基上筛选转基因后代,结合目标基因和标记基因的PCR验证,获得了超表达的转基因拟南芥。利用T_3代转基因幼苗进行耐盐性分析。研究表明,转SpSOS1基因的拟南芥在50 mmol/L NaCl的培养基中发芽率在80%以上,明显高于野生型拟南芥的45%。转SpSOS1基因拟南芥幼苗的耐盐性明显提高,在100 mmol/L NaCl条件下能正常生长,具有较高的生物量、根长和侧根数,而野生型拟南芥在50 mmol/L NaCl的胁迫下明显萎蔫、失绿、甚至死亡。说明过表达SpSOS1基因显著提高了拟南芥的耐盐性。
[Abstract]:Na~ / H- reverse transporter SOS1 plays an important role in salt tolerance in plants. In this study, the cell membrane Na~ / H- reverse transporter gene SpSOS1 from the halophytic plant sea horse teeth was constructed into the plant double expression vector pCAMBIA1304, and the SpSOS1 gene was transferred into Arabidopsis thaliana by Agrobacterium tumefaciens. Transgenic progenies were screened on MS medium containing 50 渭 g/mL hygromycin B, and superexpressed Arabidopsis thaliana was obtained by PCR verification of target gene and marker gene. The salt tolerance of transgenic seedlings of T _ 3 generation was analyzed. The results showed that the germination rate of Arabidopsis thaliana transgenic with SpSOS1 gene was more than 80% in 50 mmol/L NaCl medium, which was significantly higher than that of wild type Arabidopsis thaliana. The salt-tolerance of transgenic Arabidopsis thaliana seedlings with SpSOS1 gene increased significantly, and could grow normally under the condition of 100 mmol/L NaCl, with high biomass, root length and lateral root number, while wild-type Arabidopsis thaliana was wilted and green under 50 mmol/L NaCl stress. Even death. The results showed that overexpression of SpSOS1 gene significantly improved the salt tolerance of Arabidopsis thaliana.
【作者单位】：海南大学农学院海南省热带生物资源可持续利用重点实验室;
【基金】：海南省科技重大专项基金(HNGDhs201502)资助
【分类号】：Q943.2

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