马蔺DFR基因的克隆及生物信息学特征分析
发布时间：2019-01-03 06:21
【摘要】：以马蔺花瓣为试材,通过转录组测序,应用PCR技术克隆马蔺花青素生物合成关键酶二羟黄酮醇-4-还原酶(dihydvroflavonol-4-reductase,DFR)基因并获得其生物信息学特征。结果表明:马蔺DFR基因全长1 427bp,共编码357个氨基酸(该基因命名为IlDFR,GenBank登录号为KY907171)。该基因在氨基酸水平上与多种植物的DFR具有较高的同源性,在系统进化上与同属的荷兰鸢尾(Iris hollandica)的DFR亲缘关系较近;预测其蛋白质相对分子质量为39.99kDa,等电点为5.89,主要由α-螺旋和β-折叠构成,定位于细胞质,属于酸性亲水不含信号肽的不稳定类蛋白质。马蔺IlDFR有一段氨基酸序列‘VTGASGYVGSWLVMKLLRDGY’与大部分物种的DFR特有相对保守的NADPH结合域非常相似,只有4个氨基酸残基有所差异。
[Abstract]:The key enzyme of Iris anthocyanin biosynthesis, dihydroflavone alcohol 4-reductase (dihydvroflavonol-4-reductase,DFR), was cloned by PCR and its bioinformatics characteristics were obtained. The results showed that the total length of DFR gene was 1 427 BP, encoding 357 amino acids (IlDFR,GenBank accession number was KY907171). This gene has high homology with DFR of many plants at amino acid level, and is closely related to DFR of (Iris hollandica) of the same genus in phylogeny. The predicted molecular weight of the protein is 39.99 kDa and the isoelectric point is 5.89. The protein is mainly composed of 伪 -helix and 尾 -fold, located in the cytoplasm, and belongs to the unstable protein with acidic hydrophilic and no signal peptide. IlDFR has a segment of amino acid sequence 'VTGASGYVGSWLVMKLLRDGY', which is similar to the relatively conserved NADPH binding domain specific to DFR in most species, with only 4 amino acid residues being different.
【作者单位】： 宁夏大学西部特色生物资源保护与利用教育部重点试验室;宁夏大学生命科学学院;
【基金】：2013年度宁夏回族自治区科技支撑计划资助项目
【分类号】：S682.19
本文编号：2399002
[Abstract]:The key enzyme of Iris anthocyanin biosynthesis, dihydroflavone alcohol 4-reductase (dihydvroflavonol-4-reductase,DFR), was cloned by PCR and its bioinformatics characteristics were obtained. The results showed that the total length of DFR gene was 1 427 BP, encoding 357 amino acids (IlDFR,GenBank accession number was KY907171). This gene has high homology with DFR of many plants at amino acid level, and is closely related to DFR of (Iris hollandica) of the same genus in phylogeny. The predicted molecular weight of the protein is 39.99 kDa and the isoelectric point is 5.89. The protein is mainly composed of 伪 -helix and 尾 -fold, located in the cytoplasm, and belongs to the unstable protein with acidic hydrophilic and no signal peptide. IlDFR has a segment of amino acid sequence 'VTGASGYVGSWLVMKLLRDGY', which is similar to the relatively conserved NADPH binding domain specific to DFR in most species, with only 4 amino acid residues being different.
【作者单位】： 宁夏大学西部特色生物资源保护与利用教育部重点试验室;宁夏大学生命科学学院;
【基金】：2013年度宁夏回族自治区科技支撑计划资助项目
【分类号】：S682.19
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