兴义鸭MEF2A基因克隆及生物信息学分析
发布时间：2019-01-04 13:53
【摘要】：为了获取鸭MEF2A基因完整编码区(CDS)序列,进一步揭示其遗传机理。本试验采用兴义鸭为研究对象,采用RT-PCR方法克隆获取MEF2A基因CDS区序列,经重组质粒测序、拼接,试验初步获得了兴义鸭MEF2A基因完整编码区序列,得到1 479 bp片段,包含起始密码子和终止密码子,编码492个氨基酸,表达蛋白分子量为53.14 k D。经序列对比,在G~(429)A、T~(661)A、A~(1423)G、A~(1426)G和G~(1467)A分别发生了单核苷酸突变,其中T~(661)A和A~(1423)G突变分别导致Cys(半胱氨酸)到Ser(丝氨酸)、Glu(谷氨酸)到Lys(赖氨酸)的改变,其他位点均属于同义突变。试验对兴义鸭MEF2A蛋白的理化性质及结构特点进行了简单分析,为下一步研究MEF2A蛋白特性及其作用机制奠定理论基础。
[Abstract]:In order to obtain the complete coding region (CDS) sequence of duck MEF2A gene, the genetic mechanism was further revealed. In this experiment, Xingyi duck was used to clone and obtain the CDS sequence of MEF2A gene by RT-PCR method. After sequencing and splicing of recombinant plasmid, the complete coding region sequence of MEF2A gene of Xingyi duck was preliminarily obtained, and 1 479 bp fragment was obtained. It contains start codon and stop codon and encodes 492 amino acids, and the molecular weight of the expressed protein is 53.14 KD. By sequence comparison, single nucleotide mutations were observed in G429A429AT66A66A ~ (1423) G ~ (1426) G and G ~ (1467) A, respectively. T661A and A1423 G mutations resulted in the changes of Cys (cysteine) to Ser (serine), Glu (glutamic acid) to Lys (lysine), respectively. The other sites were synonymous mutations. The physical and chemical properties and structural characteristics of Xingyi duck MEF2A protein were analyzed in order to lay a theoretical foundation for the further study of the characteristics of MEF2A protein and its mechanism of action.
【作者单位】： 贵州大学动物科学学院;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;
【基金】：教育部科学技术研究重点项目“贵州省地方鸭品种屠宰性状相关基因的克隆与SNPs检测”(项目号:211168);鸭MEF2基因家族的克隆与组织表达规律的研究[黔科合LH字(2015)7677号]共同资助
【分类号】：S834
本文编号：2400375
[Abstract]:In order to obtain the complete coding region (CDS) sequence of duck MEF2A gene, the genetic mechanism was further revealed. In this experiment, Xingyi duck was used to clone and obtain the CDS sequence of MEF2A gene by RT-PCR method. After sequencing and splicing of recombinant plasmid, the complete coding region sequence of MEF2A gene of Xingyi duck was preliminarily obtained, and 1 479 bp fragment was obtained. It contains start codon and stop codon and encodes 492 amino acids, and the molecular weight of the expressed protein is 53.14 KD. By sequence comparison, single nucleotide mutations were observed in G429A429AT66A66A ~ (1423) G ~ (1426) G and G ~ (1467) A, respectively. T661A and A1423 G mutations resulted in the changes of Cys (cysteine) to Ser (serine), Glu (glutamic acid) to Lys (lysine), respectively. The other sites were synonymous mutations. The physical and chemical properties and structural characteristics of Xingyi duck MEF2A protein were analyzed in order to lay a theoretical foundation for the further study of the characteristics of MEF2A protein and its mechanism of action.
【作者单位】： 贵州大学动物科学学院;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;
【基金】：教育部科学技术研究重点项目“贵州省地方鸭品种屠宰性状相关基因的克隆与SNPs检测”(项目号:211168);鸭MEF2基因家族的克隆与组织表达规律的研究[黔科合LH字(2015)7677号]共同资助
【分类号】：S834
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