布氏田鼠GnRH基因克隆及不同组织和发育阶段的基因表达特征

发布时间：2019-01-08 17:23

【摘要】：促性腺激素释放激素(GnRH)由GnRH编码,是调节动物繁殖活动的核心神经内分泌物质。布氏田鼠Lasiopodomys brandtii是我国内蒙古东部草原区的害鼠之一,具有明显季节性繁殖特征,但其繁殖调控机制仍未完全明确。本研究克隆了来自布氏田鼠下丘脑的GnRH cDNA序列,使用实时荧光定量技术检测了不同组织、不同年龄阶段GnRH mRNA水平。结果表明,克隆获得GnRH cDNA序列497 bp,包含开放阅读框273 bp,编码90个氨基酸和1个终止密码子的GnRH前体。DNA序列比对和氨基酸序列同源性分析表明,布氏田鼠下丘脑GnRH基因属于Ⅰ型,与橙腹田鼠Microtus ochrogaste GnRH1相似性最高。GnRH mRNA在下丘脑、垂体、睾丸、肾上腺、肠、膀胱均有表达。雄鼠血清睾酮水平和雌鼠血清雌二醇水平在出生后8周、36周、80周处于高水平,显著高于4周龄鼠,但除80周龄鼠下丘脑GnRH表达量处于高水平,8周龄、36周龄鼠GnRH表达量与4周龄鼠差异均无统计学意义。推测4周龄鼠下丘脑GnRH受GnRH调节剂的中心抑制,而8周龄、36周龄鼠下丘脑GnRH受性类固醇介导的反馈抑制调控,当年龄增加至80周龄,性类固醇介导的反馈抑制消失或者应答时间延长。本研究结果为探究布氏田鼠繁殖调控规律提供了更多基础资料。
[Abstract]:Gonadotropin releasing hormone (GnRH), encoded by GnRH, is the core neuroendocrine substance regulating animal reproduction. Lasiopodomys brandtii is one of the pests in the eastern grassland of Inner Mongolia, which has obvious seasonal reproduction characteristics, but its reproductive regulation mechanism is not completely clear. In this study, GnRH cDNA sequences from the hypothalamus of voles brucelis were cloned, and GnRH mRNA levels in different tissues and ages were detected by real-time fluorescence quantitative technique. The results showed that the cloned GnRH cDNA sequence 497 bp, contained 90 amino acids and a stop codon GnRH precursor encoded by open reading frame 273 bp,. DNA sequence alignment and amino acid sequence homology analysis showed that, The GnRH gene in the hypothalamus of the vole belongs to type 鈪，

本文编号：2404906