基于转录组的芒果MYB家族基因的鉴定及分析

发布时间：2019-01-11 14:51

【摘要】：MYB家族作为植物中较大的转录因子家族之一,参与调控植物的多种生理活动。本研究基于芒果果实转录组测序结果,鉴定出71个MYB家族蛋白,其中包含1个4R-MYB蛋白、3个R1R2R3-MYB蛋白、60个R2R3-MYB蛋白和7个MYB相关蛋白。进化树及基序分析表明:除个别蛋白外,相同类型的MYB蛋白均聚在一起,且相近分支的MYB蛋白具有相同或相似的基序。60个全长MYB家族蛋白中,大部分为不稳定蛋白,且均为不含跨膜结构和信号肽的亲水性蛋白,亚细胞定位分析均定位于细胞核。进化树分析发现芒果R2R3-MYB蛋白与拟南芥有较高的保守性。R2R3-MYB蛋白保守域分析发现,R2和R3结构域均有多个氨基酸保守不变。GO分析发现芒果R2R3-MYB蛋白共注释到生物学过程、细胞组分和分子功能3大类功能的15个亚类。
[Abstract]:As one of the larger transcription factor families, MYB family is involved in the regulation of plant physiological activities. In this study, 71 MYB family proteins including 1 4R-MYB protein, 3 R1R2R3-MYB protein, 60 R2R3-MYB protein and 7 MYB related proteins were identified based on the results of transcriptome sequencing of mango fruit. Phylogenetic tree and motif analysis showed that the same type of MYB proteins were clustered together except for individual proteins, and the MYB proteins with similar branches had the same or similar motifs. Most of the 60 full-length MYB family proteins were unstable proteins. All of them were hydrophilic proteins without transmembrane structure and signal peptide. Subcellular localization analysis was located in the nucleus. Phylogenetic tree analysis showed that mango R2R3-MYB protein was highly conserved with Arabidopsis thaliana. R2R3-MYB protein conserved domain analysis showed that mango R2R3-MYB protein was conserved with Arabidopsis thaliana. There were many amino acids conserved in R2 and R3 domains. GO analysis showed that Mango R2R3-MYB protein was elucidated into 15 subclasses of biological processes, cellular components and molecular functions.
【作者单位】：中国热带农业科学院南亚热带作物研究所农业部热带果树生物学重点实验室;
【基金】：农业行业科研专项子项目(No.201203092-3) 广东省自然科学基金-粤西北创新人才专项(No.2016A030307006)
【分类号】：S667.7

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