NUP88基因变化对乳腺癌细胞系BT-20增殖侵袭生物学行为的影响

发布时间：2019-01-12 10:31

【摘要】：目的:探讨NUP88基因表达量升高或降低对乳腺癌细胞系BT-20细胞增殖能力、凋亡能力与侵袭能力的影响。方法:构建NUP88重组腺病毒表达载体以及NUP88 RNA干扰腺病毒载体,分别转染乳腺癌BT-20细胞获得NUP88过表达BT-20细胞以及NUP88低表达BT-20细胞并检测NUP88 mRNA和蛋白表达情况,随后通过CCK-8检测各组BT-20细胞增殖能力,通过流式双染检测各组BT-20细胞凋亡情况及通过Transwell侵袭实验检测各组BT-20细胞侵袭能力,并通过Western blot检测凋亡和侵袭相关蛋白表达变化。结果:成功获得NUP88 mRNA及蛋白高表达和低表达BT-20细胞;NUP88基因过表达导致细胞增殖能力和侵袭细胞数量显著高于正常BT-20细胞水平,而凋亡率则降低(P0.05);NUP88基因低表达导致细胞增殖能力和侵袭细胞数量显著低于正常BT-20细胞水平,而凋亡率则升高(P0.05);NUP88基因过表达导致抗凋亡蛋白Bcl-2和黏附蛋白β-catenin水平显著高于正常BT-20细胞水平,而促凋亡蛋白Bax和黏附蛋白E-cadherin显著低于正常BT-20细胞水平(P0.05);NUP88基因低表达导致Bcl-2和β-catenin水平显著低于正常BT-20细胞水平,而Bax和E-cadherin显著高于正常BT-20细胞水平(P0.05)。结论:NUP88基因通过调控凋亡相关蛋白Bax与Bcl-2和黏附蛋白E-cadherin与β-catenin水平调控BT-20细胞的增殖、凋亡和侵袭能力。
[Abstract]:Aim: to investigate the effects of increased or decreased NUP88 gene expression on the proliferation, apoptosis and invasion of breast cancer cell line BT-20. Methods: NUP88 recombinant adenovirus expression vector and NUP88 RNA interfering adenovirus vector were constructed, and NUP88 overexpression BT-20 cells and NUP88 low expression BT-20 cells were obtained by transfection into breast cancer BT-20 cells, respectively, and NUP88 mRNA and protein expression were detected. Then the proliferation of BT-20 cells in each group was detected by CCK-8, the apoptosis of BT-20 cells in each group was detected by flow double staining, and the invasiveness of BT-20 cells in each group was detected by Transwell invasion assay. The expression of apoptotic and invasive-associated proteins was detected by Western blot. Results: overexpression and low expression of NUP88 mRNA and protein in BT-20 cells were successfully obtained, and overexpression of NUP88 gene resulted in significantly higher cell proliferation and number of invasive cells than in normal BT-20 cells, but decreased apoptosis rate (P0.05). The low expression of NUP88 gene resulted in the cell proliferation ability and the number of invasive cells were significantly lower than the normal BT-20 cell level, while the apoptosis rate was increased (P0.05). Overexpression of NUP88 gene resulted in the levels of anti-apoptotic protein Bcl-2 and adhesion protein 尾-catenin were significantly higher than those of normal BT-20 cells, while apoptotic protein Bax and adhesion protein E-cadherin were significantly lower than those of normal BT-20 cells (P0.05). Low expression of NUP88 gene resulted in significantly lower levels of Bcl-2 and 尾-catenin than those of normal BT-20 cells, while Bax and E-cadherin levels were significantly higher than those of normal BT-20 cells (P0.05). Conclusion: NUP88 gene regulates the proliferation, apoptosis and invasion of BT-20 cells by regulating the levels of apoptosis-related protein Bax and Bcl-2 and adhesion protein E-cadherin and 尾-catenin.
【作者单位】：浙江大学医学院病理学与病理生理学系;临安市人民医院病理科;
【分类号】：R737.9

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