表皮生长因子受体基因表达对胃癌分期的影响及诱导胃癌细胞增殖凋亡的机制

发布时间：2019-01-14 14:26

【摘要】：目的探讨表皮生长因子受体(EGFR)基因表达对胃癌分期的影响及诱导胃癌细胞增殖凋亡的机制。方法应用免疫组化法检测100例胃癌组织中EGFR基因的表达,分析其与临床病理特征的关系。培养胃癌细胞株SGC-7901,实验分为空白对照组、空载体组、EGFR-siRNA组,3组细胞转染12、24、48、72 h后,CCK8检测EGFR基因对细胞增殖的影响;流式细胞仪检测EGFR基因对细胞凋亡的影响;Western印迹检测凋亡相关蛋白的表达。结果 100例胃癌组织中,EGFR表达阳性41例,对照组无阳性表达,差异有统计学意义(P0.01);胃癌分化程度、浸润程度、淋巴转移中EGFR的表达差异显著(P0.05),TNM分期与EGFR的表达差异极显著(P0.01);随着时间的延长,3组细胞的增殖率均上升,EGFR-siRNA组增殖较缓慢,24 h的增殖率与空白对照组和空载体组比较显著降低(P0.05),48 h和72 h的增殖率与空白对照组和空载体组比较显著降低(P0.01);3组细胞转染48 h后,EGFR-siRNA组细胞的凋亡率显著低于空白对照组和空载体组(P0.01);空白对照组和空载体组细胞中Bcl-2、Bax、酶切Caspase3 3个蛋白表达均无显著差异(P0.05),EGFR-siRNA组细胞中Bax、酶切Caspase3蛋白表达显著高于空白对照组和空载体组,Bcl-2蛋白表达显著低于空白对照组和空载体组(P0.01)。结论 EGFR基因的表达参与胃癌生长、侵袭、转移和分化过程,下调EGFR基因的表达能抑制胃癌细胞株SGC-7901增殖,促进细胞凋亡,凋亡可能与Bcl-2、Bax、Caspase3的调控有关。
[Abstract]:Objective to investigate the effect of epidermal growth factor receptor (EGFR) gene expression on gastric cancer staging and the mechanism of inducing proliferation and apoptosis of gastric cancer cells. Methods Immunohistochemical method was used to detect the expression of EGFR gene in 100 cases of gastric cancer and its relationship with clinicopathological features was analyzed. Gastric cancer cell line SGC-7901, was divided into three groups: blank control group, empty vector group, EGFR-siRNA group, and 3 groups. After transfection of 12 ~ 24N ~ 48 ~ 72 h, CCK8 was used to detect the effect of EGFR gene on cell proliferation, flow cytometry was used to detect the effect of EGFR gene on cell apoptosis. The expression of apoptosis-related protein was detected by Western blot. Results among the 100 cases of gastric cancer, 41 cases were positive for EGFR, while no positive expression was found in the control group (P0.01). The expression of EGFR in lymph node metastasis and differentiation degree of gastric cancer was significantly different (P0.05 between), TNM stage and EGFR expression (P0.01). With the prolongation of time, the proliferation rate of the three groups increased, and the proliferation rate of EGFR-siRNA group was slower than that of blank control group and empty carrier group (P0.05), and the proliferation rate of 24 h group was significantly lower than that of blank control group and empty carrier group (P0.05). The proliferation rate of 48 h and 72 h was significantly lower than that of blank control group and empty carrier group (P0.01). The apoptosis rate of EGFR-siRNA group was significantly lower than that of blank control group and empty vector group 48 h after transfection (P0.01). There was no significant difference between blank control group and empty vector group in the expression of Bcl-2,Bax, enzyme digested Caspase3 protein (P0.05). The expression of Bax, enzyme digested Caspase3 protein in EGFR-siRNA group was significantly higher than that in blank control group and empty vector group. The expression of Bcl-2 protein was significantly lower than that of blank control group and empty vector group (P0.01). Conclusion the expression of EGFR gene is involved in the process of growth, invasion, metastasis and differentiation of gastric cancer. Down-regulating the expression of EGFR gene can inhibit the proliferation of gastric cancer cell line SGC-7901 and promote apoptosis, which may be related to the regulation of Bcl-2,Bax,Caspase3.
【作者单位】：重庆市急救医疗中心病理科;西南医科大学附属第一医院病理科;
【基金】：泸州医学院自然科学基金青年基金项目(No.2013ZRQN031)
【分类号】：R735.2

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