含反药组合的海藻玉壶汤中海藻不同品种与甘草加减应用对甲状腺肿大大鼠肝脏CYP450酶基因与蛋白表达的影响

发布时间：2019-01-21 16:01

【摘要】：目的:探讨含反药组合的海藻玉壶汤中海藻不同品种与甘草加减应用对甲状腺肿大大鼠肝脏CYP450酶基因与蛋白表达的影响。方法:将大鼠随机分为空白组、模型组、阳性药组、海蒿子全方组、羊栖菜全方组、海蒿子去甘草组、羊栖菜去甘草组、去海藻组、去海藻甘草组,除空白组外其余各组灌服丙硫氧嘧啶复制甲状腺肿大模型,以优甲乐作为阳性对照,各给药组分别给予相应药物。检测各组大鼠CYP2E1、CYP3A1、CYP3A2mRNA表达及CYP2E1蛋白表达。结果:与空白组比较,模型组CYP2E1/3A2 mRNA表达明显升高(P0.05),CYP3A1 mRNA表达、CYP2E1蛋白表达有升高趋势;阳性药组CYP3A1 mRNA表达明显升高(P0.05)。与模型组比较,阳性药组、海蒿子全方组、羊栖菜全方组CYP2E1 mRNA表达明显降低(P0.05);羊栖菜全方组、去海藻组CYP3A2 mRNA表达明显降低(P0.05)。结论:丙硫氧嘧啶所致甲状腺肿大大鼠肝脏CYP2E1/3A1/3A2 mRNA表达增强,可能会对肝脏内的前毒物和前致癌物有一定的诱导作用,并且促进药物代谢吸收,从而引起肝损伤;在这一病理状态下,海藻玉壶汤(海蒿子/羊栖菜)全方对增强的相关基因表达有回调作用,并且显示出对相关蛋白表达的抑制作用,从而抑制肝脏内的前毒物,抑制药物吸收,发挥保护肝功能的作用。
[Abstract]:Aim: to investigate the effects of different seaweed varieties and liquorice on the expression of CYP450 gene and protein in the liver of rats with thyroid enlargement. Methods: rats were randomly divided into blank group, model group, positive drug group, whole prescription group, licorice group and seaweed group. The model of goiter was made by oral administration of prothiouracil in all groups except the blank group. The expression of CYP2E1,CYP3A1,CYP3A2mRNA and CYP2E1 protein were detected. Results: compared with the blank group, the expression of CYP2E1/3A2 mRNA was significantly higher in the model group (P0.05), the expression of CYP3A1 mRNA and CYP2E1 protein was higher than that of the control group (P0.05), and the expression of CYP3A1 mRNA in the positive drug group was significantly higher (P0.05). Compared with the model group, the expression of CYP2E1 mRNA was significantly decreased in the positive drug group, Artemisia artemisia group and whole prescription group (P0.05); the CYP3A2 mRNA expression in the whole prescription group and seaweed group was significantly decreased (P0.05). Conclusion: the increased expression of CYP2E1/3A1/3A2 mRNA in the liver of rats induced by propylthiouracil may induce pretoxic and precancerous substances in the liver, and promote the absorption of drug metabolism, which may lead to liver injury. In this pathological state, the whole prescription of seaweed jug (Artemisia annua / Sargassum chinensis) has a callback on the enhanced expression of related genes, and has shown an inhibitory effect on the expression of related proteins, thereby inhibiting the pretoxic substances in the liver. Inhibition of drug absorption, play a role in protecting liver function.
【作者单位】：北京中医药大学;
【基金】：国家自然科学基金项目(No.81573630)~~
【分类号】：R285.5

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