中国甘薯双生病毒外壳蛋白基因分子变异及遗传多样性分析

发布时间：2019-01-21 16:56

【摘要】：甘薯双生病毒是中国甘薯上一类重要病毒,其外壳蛋白(coat protein,CP)基因具有介体传毒、基因包被等多功能。揭示其分子变异,可以为病毒防治及育种工作提供理论依据。利用PCR方法扩增获得了24个甘薯双生病毒分离物的cp基因,采用SDT、Dna SP、RDP3、MEGA等软件对其进行分子变异分析。结果表明:24个分离物核苷酸序列均为765个碱基,一致率为88.3%~100%,编码254个氨基酸,一致率为94.5%~100%。中国甘薯双生病毒分离物系统进化树分为2个组,组I包括大部分分离物及本研究获得的22个分离物,又分为了2个亚组;组Ⅱ含有29个分离物,包括本研究中的XU8_2和XU8_3,这2个分离物与其他分离物可能为不同的种。非同义突变与同义突变比值大于1,说明cp基因处于正向选择即多样化选择。错配分布图除亚组1相对比较平滑外,其余组或亚组分离物均为多峰锯齿状,表明除亚组1种群呈突然暴发的状态外,其他组或亚种群已经存在很长时间,处于动态平衡状态。
[Abstract]:Sweet potato Gemini virus is one of the most important viruses in Chinese sweet potato. Its coat protein (coat protein,CP) gene has many functions such as virus transmission and gene coating. Revealing its molecular variation can provide theoretical basis for virus control and breeding. The cp gene of 24 isolates of sweet potato twin virus was amplified by PCR, and its molecular variation was analyzed by SDT,Dna SP,RDP3,MEGA and other software. The results showed that the nucleotide sequences of 24 isolates were all 765 bases, and the consistency rate was 88.3100 and encoded 254 amino acids. The phylogenetic tree of Chinese sweet potato twin virus isolates was divided into 2 groups. Group I consisted of most of the isolates and 22 isolates obtained in this study. Group II contains 29 isolates, including XU8_2 and XU8_3, which may be different species from other isolates. The ratio of nonsynonymous mutation to synonymous mutation is greater than 1, which indicates that the cp gene is in a positive selection, that is, diversity selection. Except for the relatively smooth subgroup 1, the other groups or subgroups were all multi-peak serrated, indicating that the other groups or subpopulations had existed for a long time and were in dynamic equilibrium, except for the sudden outbreak of subgroup 1 population.
【作者单位】：江苏徐淮地区徐州农业科学研究所农业部甘薯生物学与遗传育种重点实验室;
【基金】：江苏省自然科学基金(BK20140230) 国家甘薯产业技术体系北方病害岗位专家项目(CARS-11-B-09)
【分类号】：S435.31

【相似文献】