钠钾ATP酶介导信号转导与相关基因参与白血病细胞增殖凋亡的研究
发布时间：2019-01-25 10:21
【摘要】：目的探讨药物蟾蜍灵(Bufalin)对T系白血病jurkat细胞的作用。方法体外培养人T系白血病的细胞株(Jurkat),以浓度0、5、25、100nmol/l药物Bufalin作用jurkat细胞株,细胞计数试剂盒(cck-8)测定jurkat抑制率,膜联蛋白(annexi)V/碘化丙啶(PI)双染后,流式细胞仪用于测量jurkat细胞的凋亡率,蛋白质印迹(Western Blotting)检测与细胞凋亡相关蛋白c-myc,p57kip2的变化及Na,K-ATPase的变化.结果cck-8检测结果显示:jurkat经蟾蜍灵处理24小时后,jurkat的细胞用蟾蜍灵药物处理后,对其增殖有明显受抑作用;流式细胞仪用来检测结果表明:蟾蜍灵可以使jurkat细胞发生凋亡;蛋白质印迹检测结果显示:不同浓度蟾蜍灵药物处理jurkat细胞24小时后与细胞凋亡相关蛋白。结论蟾蜍灵可以使jurkat细胞株发生凋亡,下调的c-myc,上调的p57kip2可能参与这一过程。
[Abstract]:Objective to investigate the effect of bufalin (Bufalin) on T-lineage leukemia jurkat cells. Methods (Jurkat), a human T lineage leukemia cell line cultured in vitro, was incubated with Bufalin at a concentration of 25100nmol / l. The inhibition rate of jurkat was measured by cell count kit (cck-8), and the double staining of (annexi) V / (PI) was performed. Flow cytometry was used to measure the apoptosis rate of jurkat cells. Western blot (Western Blotting) was used to detect the changes of apoptosis related protein c-mycu p57kip2 and Na,K-ATPase. Results the results of cck-8 test showed that the proliferation of jurkat cells treated with bufalin for 24 hours was significantly inhibited after treated with bufalin. The results of flow cytometry showed that bufalin could induce apoptosis in jurkat cells, and Western blot analysis showed that different concentrations of bufalin were associated with apoptosis in jurkat cells after 24 hours of treatment with different concentrations of bufalin. Conclusion Bufalin can induce apoptosis of jurkat cell line and down-regulate c-myc, and up-regulated p57kip2 may participate in this process.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R733.7
本文编号：2415044
[Abstract]:Objective to investigate the effect of bufalin (Bufalin) on T-lineage leukemia jurkat cells. Methods (Jurkat), a human T lineage leukemia cell line cultured in vitro, was incubated with Bufalin at a concentration of 25100nmol / l. The inhibition rate of jurkat was measured by cell count kit (cck-8), and the double staining of (annexi) V / (PI) was performed. Flow cytometry was used to measure the apoptosis rate of jurkat cells. Western blot (Western Blotting) was used to detect the changes of apoptosis related protein c-mycu p57kip2 and Na,K-ATPase. Results the results of cck-8 test showed that the proliferation of jurkat cells treated with bufalin for 24 hours was significantly inhibited after treated with bufalin. The results of flow cytometry showed that bufalin could induce apoptosis in jurkat cells, and Western blot analysis showed that different concentrations of bufalin were associated with apoptosis in jurkat cells after 24 hours of treatment with different concentrations of bufalin. Conclusion Bufalin can induce apoptosis of jurkat cell line and down-regulate c-myc, and up-regulated p57kip2 may participate in this process.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R733.7
【参考文献】
相关期刊论文 前1条
1 熊安秀;王敏;金润铭;白燕;林雯;;哇巴因诱导Jurkat细胞凋亡与胱冬酶-3及bcl-2基因家族的关系[J];中国实验血液学杂志;2006年05期
，本文编号：2415044
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