云南蓝舌病病毒1型毒株M6基因序列分析

发布时间：2019-02-09 08:11

【摘要】：为了解云南蓝舌病病毒(Bluetongue virus,BTV)1型M6基因流行株的遗传变异及其与国内外流行病毒的遗传进化关系,试验从细胞培养物中分别提取4株云南分离株BTV-1(Y863、SZ120169、6-12和7-12)RNA,用M6基因特异引物进行RT-PCR扩增和测序,采用生物信息学软件对获得的M6基因编码区序列进行核苷酸、氨基酸同源性比对及遗传进化分析。结果表明,分别获得4株云南分离株BTV-1 M6基因1 763bp序列;4株云南分离株BTV-1核苷酸同源性在95.2%~99.9%之间,氨基酸同源性在97.6%~99.8%之间,1979年师宗分离的Y863病毒毒株与2012年师宗(SZ120169)、2013年江城(6-12、7-12)分离的3株病毒毒株核苷酸同源性分别为95.5%、95.2%和95.2%,氨基酸同源性分别为97.6%、98.4%和98.2%,而近两年(2012、2013)分离病毒核苷酸和氨基酸同源性较高,分别在96.9%~99.9%和99.1%~99.8%之间;遗传进化分析发现,4株云南分离株BTV-1为Eastern基因群病毒,它们之间核苷酸和氨基酸同源性分别为95.2%~99.9%和97.6%~99.8%;进一步分析发现4株云南分离株BTV-1与希腊及澳大利亚BTV-1型毒株亲缘关系较近,核苷酸和氨基酸同源性分别为90.4%~95.6%和95.1%~99.1%,而与地中海国家(意大利、法国、阿尔及利亚、摩洛哥和突尼斯)和南非毒株关系较远,核苷酸和氨基酸同源性分别在83.8%和95.7%以下。4株云南分离株BTV-1属于Eastern基因群病毒,云南分离株BTV-1 M6基因在自然进化中发生遗传变异缓慢,该基因可以用来进行BTV-1基因群分布及毒株的地理区域来源相关的研究。
[Abstract]:In order to understand the genetic variation of Yunnan bluetongue virus (Bluetongue virus,BTV) type 1 M6 gene and its relationship with the domestic and foreign epidemic viruses, four Yunnan strain BTV-1 (Y863) were isolated from cell cultures. SZ120169,6-12 and 7-12) RNA, were amplified by M6 gene specific primers and sequenced. Nucleotide, amino acid homology and genetic evolution were analyzed by bioinformatics software. The results showed that BTV-1 M6 gene 1 763bp sequence was obtained from 4 Yunnan isolates. The nucleotide homology and amino acid homology of BTV-1 and amino acid were 95.2% and 99.8%, respectively. The Y863 virus strains isolated in 1979 and 2012 had the same nucleotide identity and amino acid homology (SZ120169), and the nucleotide homology of Y863 virus isolated from Yunnan was 99.9%, and the amino acid homology was 99.8%. The nucleotide homology and amino acid homology of three virus strains isolated from Jiangcheng in 2013 were 95.5% and 95.2%, respectively, and the amino acid homology were 97.6% and 98.2%, respectively. The nucleotide and amino acid homology of virus isolated in the past two years (2012 / 2013) was between 99.9% and 99.8%, respectively, and the homology of nucleotide and amino acid was 99.9% and 99.1%, respectively. The results of genetic evolution analysis showed that BTV-1 was a Eastern gene group virus, and the nucleotide and amino acid homology between them were 95.299.9% and 97.6%, respectively. Further analysis showed that the four Yunnan isolates were closely related to the BTV-1 strains of Greece and Australia. The homology of nucleotide and amino acid were 90.4% and 95.6%, respectively, and 95.1% and 99.1%, respectively. They are more distant from the Mediterranean countries (Italy, France, Algeria, Morocco and Tunisia) and from South Africa. The nucleotide and amino acid homology were below 83.8% and 95.7%, respectively. 4 strains of Yunnan isolate BTV-1 belong to Eastern gene group virus. The genetic variation of BTV-1 M6 gene in Yunnan isolate is slow in natural evolution. This gene can be used to study the distribution of BTV-1 gene group and the geographical source of the virus strain.
【作者单位】：云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室;
【基金】：云南省应用基础研究计划(青年项目)(2012FD082) 国家公益性(农业)行业专项(201303035)
【分类号】：S852.65

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