干旱胁迫棉花转录组DNA损伤修复相关基因的分析

发布时间：2019-02-12 20:59

【摘要】：【目的】研究干旱胁迫下,棉花DNA损伤修复基因的表达情况,从整体水平探讨棉花DNA损伤修复相关基因表达与抗旱的相关性。【方法】用2.5%PEG6000处理棉花幼苗,利用RNA-Seq技术对干旱胁迫下棉花幼苗的转录组进行测序。【结果】从棉花干旱胁迫响应的转录组中,筛选获得差异表达的DNA损伤修复相关基因共51个,其中差异表达的上调基因23个,差异表达的下调基因28个,干旱胁迫能够影响棉花DNA损伤修复相关基因的表达。选取4个差异基因进行生物信息学分析及qRT-PCR验证,HMGB1、rec A1、UDGs和GMP synthase基因的表达变幅有一定的差异,但基因的表达趋势一致,棉花转录组测序结果通过qRTPCR验证是可靠的。【结论】DNA损伤修复相关基因可能与干旱胁迫有一定的相关性,干旱胁迫能够影响棉花DNA损伤修复相关基因的表达。
[Abstract]:[objective] to study the expression of DNA damage repair genes in cotton under drought stress, and to explore the correlation between the expression of DNA damage repair genes and drought resistance in cotton seedlings. [methods] Cotton seedlings were treated with 2.5%PEG6000. The transcriptome of cotton seedlings under drought stress was sequenced by RNA-Seq technique. [results] 51 differentially expressed genes related to DNA damage repair were screened from the transcriptional groups of cotton drought stress response. Among them, 23 genes were up-regulated and 28 genes were down-regulated. Drought stress could affect the expression of genes related to DNA damage and repair in cotton. Four differentially expressed genes were selected for bioinformatics analysis and qRT-PCR verification. There were some differences in the expression of HMGB1,rec A1UDGs and GMP synthase genes, but the trend of gene expression was the same. [conclusion] DNA damage repair related genes may be related to drought stress, drought stress can affect the expression of cotton DNA damage repair related genes.
【作者单位】：新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室;
【基金】：国家自然科学基金-新疆联合基金重点项目“棉花水分高效利用的关键基因的挖掘及抗旱种质材料的创制”(U1303282)~~
【分类号】：S562

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