短时热激后家蚕对核型多角体病毒的抗性及相关抗菌肽和热激蛋白基因表达水平的变化
发布时间：2019-02-13 01:44
【摘要】：【目的】探讨短时热激对家蚕Bombyx mori抵抗核型多角体病毒能力的影响,为深入研究家蚕热激响应的抗病毒免疫机制提供参考。【方法】家蚕5龄幼虫42℃热激15 min后,经口喂食家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,Bm NPV)(5.4×106PIBs/头)后正常饲养,观察热激对家蚕感染病毒的影响;同时,利用实时荧光定量RT-PCR技术检测热激后7类抗菌肽主基因(Bmcec D,Bmmor,Bmglve2,Bmdefe B,Bmatta1,Bmenbo2和Bmlebo3)以及6种热激蛋白基因(Bmhsp90,Bmhsp70,Bmhsp40,Bmhsp19.9,Bmhsp21.4和Bmhsp25.4)在家蚕5龄幼虫中肠组织中的表达变化;利用MEGA 5.0软件对家蚕所有热激蛋白进行系统进化分析。【结果】与未进行热激处理相比,短时42℃热激能够明显提高感染Bm NPV的家蚕5龄幼虫的存活率。实时荧光定量RT-PCR结果表明,与未热激处理相比,热激处理后感染Bm NPV的家蚕5龄幼虫中肠组织中Bmglve2和Bmhsp25.4的表达量显著上升。另外,系统进化分析显示,小分子热激蛋白Bm HSP25.4在进化上特殊,单独与大分子热激蛋白(Bm HSP90,Bm HSP70和Bm HSP40)聚为一类。【结论】短时热激能够提高家蚕对Bm NPV的抗性,热激蛋白基因Bmhsp25.4和抗菌肽基因Bmglve2可能在家蚕热激响应的抗病毒免疫中发挥关联功能。
[Abstract]:[objective] to investigate the effect of short term heat shock on the ability of silkworm Bombyx mori to resist nuclear polyhedrosis virus, and to provide a reference for further study on the antiviral mechanism of heat shock response of Bombyx mori. [methods] the 5th instar larvae of Bombyx mori were exposed to heat shock for 15 min at 42 鈩，
本文编号：2421043
[Abstract]:[objective] to investigate the effect of short term heat shock on the ability of silkworm Bombyx mori to resist nuclear polyhedrosis virus, and to provide a reference for further study on the antiviral mechanism of heat shock response of Bombyx mori. [methods] the 5th instar larvae of Bombyx mori were exposed to heat shock for 15 min at 42 鈩，
本文编号：2421043
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