越橘类黄酮化合物转运相关基因MATE的克隆与表达分析
发布时间：2019-02-14 10:19
【摘要】：植物中多药和毒性化合物外排(MATE)转运蛋白参与类黄酮在液泡中积累的过程。以高丛越橘品种"北卫"为试验材料,应用染色体步移和cDNA末端快速克隆技术克隆MATE转运蛋白基因的cDNA全长序列,命名为VcMATE6,序列提交到GenBank,登录号为KF875437。序列分析表明VcMATE6的cDNA全长1 557 bp,编码518个氨基酸,推测的蛋白相对分子质量为56.39 ku,理论等电点为6.12。多重序列比对显示VcMATE6与MATE型类黄酮转运蛋白有较高的同源性。聚类分析表明VcMATE6与MtMATE2、MlMTP77、AtTT12、VvAM1等一类植物典型的MATE型类黄酮转运蛋白聚为一类。VcMATE6具有2个MATE家族特有的Mat E结构域,TMHMM在线软件预测VcMATE6含有12个跨膜螺旋。实时荧光定量PCR分析表明,VcMATE6基因在越橘各器官和果实发育各阶段均有表达,但表达量存在差异,蓝果种子中表达量最高,极显著高于其他器官以及蓝果的果皮和果肉组织,其次为根,果实发育过程中VcMATE6表达量随果实成熟度表现出增加的趋势。
[Abstract]:The efflux of (MATE) transporter from plant multidrug and toxic compounds is involved in the accumulation of flavonoids in vacuoles. The full-length cDNA sequence of MATE transporter gene was cloned by chromosomal walking and rapid cloning of cDNA terminal using "Beiwei", a variety of blueberry cultivars with high density, named VcMATE6, sequence and GenBank, accession number KF875437.. Sequence analysis showed that the cDNA length of VcMATE6 was 1 557 bp, encoding 518 amino acids, and the predicted molecular weight of the protein was 56. 39 ku, with a theoretical isoelectric point of 6. 12. Multiple sequence alignment showed high homology between VcMATE6 and MATE type flavonoid transporter. Cluster analysis showed that some typical MATE flavonoid transporters, such as VcMATE6 and MtMATE2,MlMTP77,AtTT12,VvAM1, were clustered into a class. VcMATE6 had two Mat E domains unique to the MATE family, and TMHMM online software predicted that VcMATE6 contained 12 transmembrane helices. Real-time fluorescence quantitative PCR analysis showed that VcMATE6 gene was expressed in all organs and fruit development stages of blueberry, but there were differences in the expression levels. The highest expression level of VcMATE6 gene was found in blueberry seeds, which was significantly higher than that in other organs and the peel and pulp tissues of blueberry. The second was root, and the expression of VcMATE6 increased with fruit maturity during fruit development.
【作者单位】： 吉林农业大学园艺学院;
【基金】：国家自然科学基金项目(31301755) 高等学校博士学科点专项科研基金项目(20132223120004) 农业部公益性行业(农业)科研专项(201103037) 人力资源和社会保障部留学人员科技活动项目择优资助项目 吉林省科技发展计划科技引导计划项目(20150414047GH)
【分类号】：S663.9
，
本文编号：2422117
[Abstract]:The efflux of (MATE) transporter from plant multidrug and toxic compounds is involved in the accumulation of flavonoids in vacuoles. The full-length cDNA sequence of MATE transporter gene was cloned by chromosomal walking and rapid cloning of cDNA terminal using "Beiwei", a variety of blueberry cultivars with high density, named VcMATE6, sequence and GenBank, accession number KF875437.. Sequence analysis showed that the cDNA length of VcMATE6 was 1 557 bp, encoding 518 amino acids, and the predicted molecular weight of the protein was 56. 39 ku, with a theoretical isoelectric point of 6. 12. Multiple sequence alignment showed high homology between VcMATE6 and MATE type flavonoid transporter. Cluster analysis showed that some typical MATE flavonoid transporters, such as VcMATE6 and MtMATE2,MlMTP77,AtTT12,VvAM1, were clustered into a class. VcMATE6 had two Mat E domains unique to the MATE family, and TMHMM online software predicted that VcMATE6 contained 12 transmembrane helices. Real-time fluorescence quantitative PCR analysis showed that VcMATE6 gene was expressed in all organs and fruit development stages of blueberry, but there were differences in the expression levels. The highest expression level of VcMATE6 gene was found in blueberry seeds, which was significantly higher than that in other organs and the peel and pulp tissues of blueberry. The second was root, and the expression of VcMATE6 increased with fruit maturity during fruit development.
【作者单位】： 吉林农业大学园艺学院;
【基金】：国家自然科学基金项目(31301755) 高等学校博士学科点专项科研基金项目(20132223120004) 农业部公益性行业(农业)科研专项(201103037) 人力资源和社会保障部留学人员科技活动项目择优资助项目 吉林省科技发展计划科技引导计划项目(20150414047GH)
【分类号】：S663.9
，
本文编号：2422117
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2422117.html
最近更新
教材专著
