密码子优化BTRCP-CypA融合基因的PCR合成
发布时间：2019-02-16 01:21
【摘要】：为了提高BTRCP-Cyp A融合基因的表达量,对基因密码子进行优化,主要是去除过多富含AT碱基以及提高GC含量。在此基础上提出一种用于基因合成的PCR法。拆分单链寡核首酸长度为59 nt左右,重叠区碱基数14~21 nt,Tm值为46~62℃。具体步骤如下:把拆分的寡核首酸组装成300~500 bp左右的中间片段,再将中间片段拼接成全长基因。结果表明利用该法法合成了BTRCP-Cyp A融合基因,并提高了表达量。因而改良的PCR法能经济、简便、高效地进行基因合成,具有良好的应用前景。
[Abstract]:In order to improve the expression of BTRCP-Cyp A fusion gene, the codon of the gene was optimized to remove the excessive amount of AT bases and increase the content of GC. On this basis, a PCR method for gene synthesis is proposed. The length of cleavage oligonucleus was about 59 nt, the base number of overlapping region was 14 ~ 21 nt,Tm and the value was 46 ~ 62 鈩，
本文编号：2423899
[Abstract]:In order to improve the expression of BTRCP-Cyp A fusion gene, the codon of the gene was optimized to remove the excessive amount of AT bases and increase the content of GC. On this basis, a PCR method for gene synthesis is proposed. The length of cleavage oligonucleus was about 59 nt, the base number of overlapping region was 14 ~ 21 nt,Tm and the value was 46 ~ 62 鈩，
本文编号：2423899
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