基于数字基因表达谱分析的茶树花不育基因挖掘

发布时间：2019-02-16 03:23

【摘要】：茶树每年都要开花结实,消耗大量的养分,导致茶树鲜叶产量减少和品质降低。了解茶树的不育机制有助于培育茶树不育品种。本研究以福鼎大白茶(父本)、佛香2号(母本)及其杂交后代(不育)茶树花为材料,利用数字基因表达谱技术对3个茶树花的c DNA文库进行差异基因表达谱分析。筛选出在父本花与子代不育花、母本花与子代不育花之间共有而在父本花与母本花之间没有的差异表达序列1219条,被认为是茶树不育性候选基因。GO功能显著性分析表明,这些基因功能中代谢过程、催化活性、水解酶活性表现为富集;KEGG代谢分析表明,差异表达基因涉及氨基酸、糖、次生代谢、植物信号传导途径以及能量代谢等过程。以植物激素信号转导通路分析发现,16个与生长素信号途径相关基因中,除5个ARF家族基因在子代不育花中上调表达外,其他的基因均下调,推测生长素信号转导是茶树花不育的重要因素。随机抽取5个基因进行实时荧光定量PCR验证,其结果与测序结果一致。本研究发现的不育候选基因可用于茶树花不育机制的深入研究和不育基因的筛选。
[Abstract]:Tea plants bloom and bear fruit every year, consuming a lot of nutrients, which results in the decrease of fresh leaf yield and quality. Understanding the mechanism of tea plant sterility is helpful to the cultivation of tea plant sterile varieties. In this study, the c DNA library of 3 tea plants were analyzed by digital gene expression technique using Fudingdabai tea (male parent), Foxiang 2 (female parent) and their hybrid progenies (sterile) as materials. 1219 differentially expressed sequences were screened between the male flower and the offspring sterile flower, the female parent flower and the offspring sterile flower, but not between the male parent flower and the female parent flower, which were considered to be the candidate genes for tea plant sterility. The analysis of GO function showed that there was no difference between the male flower and the female parent flower. The metabolic process, catalytic activity and hydrolase activity of these genes were enriched; KEGG metabolic analysis showed that differentially expressed genes were involved in amino acid, sugar, secondary metabolism, plant signal transduction pathway and energy metabolism. Plant hormone signal transduction pathway analysis showed that all 16 genes associated with auxin signaling pathway were down-regulated except 5 ARF family genes which were up-regulated in sterile flowers. It is inferred that auxin signal transduction is an important factor of tea flower sterility. Five genes were randomly selected for real-time fluorescence quantitative PCR validation, and the results were consistent with the sequencing results. The candidate genes can be used to study the mechanism of tea flower sterility and to screen sterile genes.
【作者单位】：云南省农业科学院茶叶研究所/云南省茶树种质资源创新与配套栽培技术工程研究中心;安徽农业大学茶树生物学与资源利用国家重点实验室;
【基金】：国家自然科学基金项目(31560220,31460216) 云南省人才培养计划项目(2015HB105) 茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20150105)资助~~
【分类号】：S571.1

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