高锌胁迫对八棱海棠幼苗锌积累、分配与9个MTP基因表达的影响

发布时间：2019-03-02 08:33

【摘要】：为了研究高锌(Zn)胁迫下苹果砧木八棱海棠(Malus robusta Rehd.)Zn积累、分配及分子响应机制,以其8叶龄实生苗为试验材料,采用溶液培养法进行不同浓度(4、25、50、100μmol·L~(-1))Zn处理。试验结果表明:过量Zn胁迫严重影响植株生长及Zn的积累与分配,随着Zn处理浓度的升高,八棱海棠幼苗根系中Zn含量显著高于茎和叶;富集系数(BAF)的增大和转运系数(TF)的减小都说明Zn在根系中大量积累,以减轻对地上部的伤害。根据对9个MTP基因的相对表达量以及相关性分析,认为在高Zn胁迫下MTP基因的表达与植株内Zn含量有密切联系。MTP1在茎、叶中显著表达,具有组织特异性。MTPC2-like的相对表达量在4μmol·L~(-1) Zn处理下与根、茎、叶的Zn含量显著正相关,而在较高浓度Zn胁迫下相关性减弱,这说明MTP基因的表达具有浓度效应。
[Abstract]:In order to study the mechanism of (Malus robusta Rehd.) Zn accumulation, distribution and molecular response in apple rootstocks under high zinc (Zn) stress, 8-leaf-old seedlings were used as experimental materials. Different concentrations (4,25,50100 渭 mol 路L ~ (- 1) Zn) were treated by solution culture method. The results showed that excessive Zn stress seriously affected plant growth and the accumulation and distribution of Zn. With the increase of Zn concentration, the content of Zn in root of Malus malus seedlings was significantly higher than that of stems and leaves. The increase of enrichment coefficient (BAF) and the decrease of transport coefficient (TF) indicated that Zn accumulated in the root system in order to reduce the damage to shoot. According to the relative expression and correlation analysis of 9 MTP genes, it was concluded that the expression of MTP gene was closely related to the content of Zn in plants under high Zn stress. The relative expression of MTPC2-like was positively correlated with the content of Zn in roots, stems and leaves under 4 渭 mol 路L ~ (- 1) Zn treatment, but weakened under higher concentration of Zn stress, which indicated that the expression of MTP gene had a concentration effect.
【作者单位】：甘肃农业大学园艺学院;
【基金】：兰州市科技局项目(2015-3-76) 甘肃省林业厅项目(GSLT2016-1)
【分类号】：S661.1

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