谷子农艺和品质性状与Waxy基因exon4-exon9区间RT-PCR分析
发布时间：2019-03-02 11:29
【摘要】：以来自不同生态区的51份谷子品种为材料,对其农艺性状、品质性状进行相关性和聚类分析,并对4份粳糯谷子材料进行Waxy基因exon4-exon9区间RT-PCR分析。其主要结果如下：(1)供试51份材料的农艺性状的变异范围为7.89%-49.60%,产量的变异范围为94.72 kg/亩-484.44kg/亩,其变异系数为21.81%。用类平均法当阈值为4时,供试材料被聚为七大类；第一类品种最多,有大乌谷等27个品种,第七类只有一个品种K97混2,但其产量是最高的。与产量呈极显著正相关的农艺指标包括节数及叶片数、穗粗、穗重、穗粒重和千粒重,各农艺指标之间呈极显著负相关的有两组,分别为穗粗与穗下节间长,穗长与穗的松紧度。(2)济0515的蛋白质含量最高,大同34的含量最低；公谷68的维生素B1含量最高为0.68mg/100g;九谷11的维生素B2含量远远高于其他品种的谷子,为0.72mg/100g;鑫谷10号和A2-2258这两个品种的谷子硒含量均在30μg/kg以上,可以作为硒相对含量较高的品种种植加工生产。供试材料中胶稠度120mm的有16个品种；46份材料为中等糊化温度；猫爪粘等3份材料属于糯质类型,白米粘谷和公谷68的直链淀粉含量为0；直链淀粉含量大于17%的非糯品种有4份；低直链淀粉类型的品种有39份。(3)供试51份谷子材料品质性状的变异范围为9.38%-128.57%,蛋白质含量的变异系数最小,维生素B2的变异系数最大。用最小距离法当阈值为10时,供试材料被聚为九类；第三类直链淀粉含量最高含有34个品种；第四类、第五类的直链淀粉含量均为O。维生素B1与维生素B2呈极显著正相关,直链淀粉与蛋白质含量呈极显著负相关。(4) exon4-exon9外显子区间序列比对表明：红粘谷(00000537)与白米粘谷的同源性为99.12%,有4个单核苷酸多态性的变异；丰田501与赤谷4号的同源性为98.9%,有5个单核苷酸多态性的变异；粳糯谷对比,红粘谷(00000537)与赤谷4号相似性为98.23%,其中红粘谷(00000537)在139、163、189、192、245、358、445和451位点依次分别为T、G、T、G、G、A、C和A,赤谷4号在上述位点的碱基分别为C、A、G、A、A、C、T和G；红粘谷(00000537)和赤谷4号的exon4- exon 9区间的cDNA序列翻译出的氨基酸序列比对,共检测到6个氨基酸变异。
[Abstract]:Using 51 millet varieties from different ecological regions as materials, the correlation and cluster analysis of agronomic and quality traits were carried out, and 4 japonica waxy millet varieties were analyzed by exon4-exon9 interval RT-PCR analysis of Waxy gene. The main results were as follows: (1) the variation range of agronomic characters and yield were 7.89% / 49.60% and 94.72 kg/-484.44kg/ mu, respectively, and the coefficient of variation was 21.81%. When the threshold value was 4, the tested materials were clustered into seven categories, the first variety was the most, there were 27 varieties such as Dawugu and so on, and in the seventh category there was only one variety K97 mixed 2, but its yield was the highest. The agronomic indexes with very significant positive correlation with yield included node number and leaf number, ear diameter, ear weight, grain weight per ear and 1000-grain weight. There were two groups with significant negative correlation between each agronomic index, namely, ear diameter and length of Internode under panicle, and there were two groups with significant negative correlation between each agronomic index, namely, ear diameter and under-panicle Internode length. (2) the protein content of Ji 0515 was the highest, that of Datong 34 was the lowest; The highest content of vitamin B1 in Ganggu 68 was 0.68mg / 100g, and the content of vitamin B2 in Jiugu 11 was much higher than that in other varieties of millet, which was 0.72mg / 100g. The content of selenium in millet of Xingu 10 and A _ 2 O _ (2258) was more than 30 渭 g / kg, which could be used as the planting and processing of the varieties with relatively high se content. Among the tested materials, 16 varieties had gel consistency 120mm, 46 materials were medium gelatinization temperature, 3 materials such as cat-claw stickiness belonged to waxy type, the amylose content of white rice sticky grain and gonggu 68 was 0, the content of amylose in white rice sticky grain and gonggu 68 was 0. There were 4 non-waxy varieties with amylose content greater than 17%. There were 39 varieties with low amylose type. (3) the variation range of quality characters of 51 millet materials was 9.38% and 128.57%, the coefficient of variation of protein content was the smallest, and the coefficient of variation of vitamin B2 was the largest. When the threshold value was 10:00, the tested materials were clustered into nine groups; the amylose content of the third type contained 34 varieties; and the amylose content of the fourth and fifth groups were O. Vitamin B1 was positively correlated with vitamin B2, amylose was negatively correlated with protein content. (4) sequence alignment of exon4-exon9 exons showed that red gluten (00000537) had 99.12% homology with white rice. There were 4 single nucleotide polymorphism variations. The homology between Toyota 501 and Chigu 4 was 98.9%, and there were 5 single nucleotide polymorphism variations. In comparison with glutinous rice, the similarity of Hongyanggu (00000537) to Chigu 4 was 98.23%, in which Hongyanggu (00000537) was T, G, A, C and A at positions 139163189, 19245358445 and 451, respectively, and that of red glutinous grain (00000537) was T, G, A, C and A, respectively. The base of Chigu 4 was C, A, G, A, C, T and G, respectively. A total of 6 amino acid variations were detected in the cDNA sequences translated from the exon4- exon 9 region of Hongyanggu (00000537) and Chigu 4. The amino acid sequences of red gluten (00000537) and Chigu 4 were compared with each other.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S515
本文编号：2433005
[Abstract]:Using 51 millet varieties from different ecological regions as materials, the correlation and cluster analysis of agronomic and quality traits were carried out, and 4 japonica waxy millet varieties were analyzed by exon4-exon9 interval RT-PCR analysis of Waxy gene. The main results were as follows: (1) the variation range of agronomic characters and yield were 7.89% / 49.60% and 94.72 kg/-484.44kg/ mu, respectively, and the coefficient of variation was 21.81%. When the threshold value was 4, the tested materials were clustered into seven categories, the first variety was the most, there were 27 varieties such as Dawugu and so on, and in the seventh category there was only one variety K97 mixed 2, but its yield was the highest. The agronomic indexes with very significant positive correlation with yield included node number and leaf number, ear diameter, ear weight, grain weight per ear and 1000-grain weight. There were two groups with significant negative correlation between each agronomic index, namely, ear diameter and length of Internode under panicle, and there were two groups with significant negative correlation between each agronomic index, namely, ear diameter and under-panicle Internode length. (2) the protein content of Ji 0515 was the highest, that of Datong 34 was the lowest; The highest content of vitamin B1 in Ganggu 68 was 0.68mg / 100g, and the content of vitamin B2 in Jiugu 11 was much higher than that in other varieties of millet, which was 0.72mg / 100g. The content of selenium in millet of Xingu 10 and A _ 2 O _ (2258) was more than 30 渭 g / kg, which could be used as the planting and processing of the varieties with relatively high se content. Among the tested materials, 16 varieties had gel consistency 120mm, 46 materials were medium gelatinization temperature, 3 materials such as cat-claw stickiness belonged to waxy type, the amylose content of white rice sticky grain and gonggu 68 was 0, the content of amylose in white rice sticky grain and gonggu 68 was 0. There were 4 non-waxy varieties with amylose content greater than 17%. There were 39 varieties with low amylose type. (3) the variation range of quality characters of 51 millet materials was 9.38% and 128.57%, the coefficient of variation of protein content was the smallest, and the coefficient of variation of vitamin B2 was the largest. When the threshold value was 10:00, the tested materials were clustered into nine groups; the amylose content of the third type contained 34 varieties; and the amylose content of the fourth and fifth groups were O. Vitamin B1 was positively correlated with vitamin B2, amylose was negatively correlated with protein content. (4) sequence alignment of exon4-exon9 exons showed that red gluten (00000537) had 99.12% homology with white rice. There were 4 single nucleotide polymorphism variations. The homology between Toyota 501 and Chigu 4 was 98.9%, and there were 5 single nucleotide polymorphism variations. In comparison with glutinous rice, the similarity of Hongyanggu (00000537) to Chigu 4 was 98.23%, in which Hongyanggu (00000537) was T, G, A, C and A at positions 139163189, 19245358445 and 451, respectively, and that of red glutinous grain (00000537) was T, G, A, C and A, respectively. The base of Chigu 4 was C, A, G, A, C, T and G, respectively. A total of 6 amino acid variations were detected in the cDNA sequences translated from the exon4- exon 9 region of Hongyanggu (00000537) and Chigu 4. The amino acid sequences of red gluten (00000537) and Chigu 4 were compared with each other.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S515
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