火力楠转录组测序与组织差异表达基因分析
发布时间：2019-03-03 13:46
【摘要】：火力楠具有重要经济价值与生态功能,但缺乏其基因组信息,限制了其分子生物学、基因功能与分子育种的相关研究。以火力楠根、茎和叶为材料,利用Illumina高通量测序技术进行转录组测序,并对得到的测序数据进行de novo组装,获得97 503条Unigenes,N50为1 010 bp、平均长度852 bp。与公共数据库进行比对,注释到NR、Swiss-Prot数据库的Unigenes分别为60 926、44 249条。将Unigenes与COG数据库比对,有42 344条Unigenes成功注释,根据功能大致分成25类;与GO数据库比对,有15 947条Unigenes获得注释,按功能可分为生物学过程、细胞组分和分子功能3大类55亚类,其中参与的生物学过程较多;以KEGG数据库参考,有26 267条Unigenes参与330条代谢途径分支,以代谢相关的途径较为集中;差异基因表达分析我们得出,根与茎的差异表达基因为2 826个,根与叶之间的差异表达基因为3 230个,这两组差异基因相对校多,除此之外茎与叶差异基因相对较少;差异基因的GO分类、GO富集以及pathway富集分析表明,主要在细胞组分、蛋白质活性、生物合成以及代谢过程等所占比例较高。这些研究结果极大地扩充了火力楠的基因资源,将有助于火力楠基因的发掘与利用、分子标记的开发及其种质资源遗传改良的研究等。
[Abstract]:Photinia officinalis has important economic value and ecological function, but the lack of genomic information limits its molecular biology, gene function and molecular breeding related research. Using root, stem and leaf as materials, the transcript sequence was sequenced by Illumina high flux sequencing technique, and the obtained sequencing data were assembled by de novo. 97,503 Unigenes,N50 were obtained as the average length of 1010 bp, and the average length of 97,503 Unigenes,N50 was 1 010 bp, and the average length of the sequence was 1 010 bp,. Compared with the common database, the Unigenes annotated to the NR,Swiss-Prot database was 60926,44,249 respectively. Comparing Unigenes with COG database, there are 42,344 successful comments of Unigenes, which are divided into 25 categories according to their functions. Compared with GO database, 15,947 Unigenes were annotated, which could be divided into biological process, cellular component and molecular function. According to KEGG database, 26,267 Unigenes were involved in 330 branches of metabolic pathway, and the metabolic related pathways were more concentrated. The differential gene expression analysis showed that there were 2 826 differentially expressed genes between root and stem and 3 230 differentially expressed genes between root and leaf. In addition, there were less differentially expressed genes between stem and leaf in the two groups. The analysis of GO classification, GO enrichment and pathway enrichment of differential genes showed that the proportion of differential genes was higher in cell composition, protein activity, biosynthesis and metabolic process. These results greatly expand the genetic resources of Photinia, which will contribute to the exploration and utilization of the gene, the development of molecular markers and the genetic improvement of germplasm resources, etc.
【作者单位】： 中国林业科学研究院热带林业研究所热带林业研究国家林业局重点实验室;
【基金】：中央公益性科研院所基金项目(RITFYWZX201506;CAFYBB2016SZ002) “十三五”国家重点研发计划项目任务(2017YFD0601101-7) 林业公益性行业科研专项(201204304-02)共同资助
【分类号】：Q943.2;S792.99
本文编号：2433750
[Abstract]:Photinia officinalis has important economic value and ecological function, but the lack of genomic information limits its molecular biology, gene function and molecular breeding related research. Using root, stem and leaf as materials, the transcript sequence was sequenced by Illumina high flux sequencing technique, and the obtained sequencing data were assembled by de novo. 97,503 Unigenes,N50 were obtained as the average length of 1010 bp, and the average length of 97,503 Unigenes,N50 was 1 010 bp, and the average length of the sequence was 1 010 bp,. Compared with the common database, the Unigenes annotated to the NR,Swiss-Prot database was 60926,44,249 respectively. Comparing Unigenes with COG database, there are 42,344 successful comments of Unigenes, which are divided into 25 categories according to their functions. Compared with GO database, 15,947 Unigenes were annotated, which could be divided into biological process, cellular component and molecular function. According to KEGG database, 26,267 Unigenes were involved in 330 branches of metabolic pathway, and the metabolic related pathways were more concentrated. The differential gene expression analysis showed that there were 2 826 differentially expressed genes between root and stem and 3 230 differentially expressed genes between root and leaf. In addition, there were less differentially expressed genes between stem and leaf in the two groups. The analysis of GO classification, GO enrichment and pathway enrichment of differential genes showed that the proportion of differential genes was higher in cell composition, protein activity, biosynthesis and metabolic process. These results greatly expand the genetic resources of Photinia, which will contribute to the exploration and utilization of the gene, the development of molecular markers and the genetic improvement of germplasm resources, etc.
【作者单位】： 中国林业科学研究院热带林业研究所热带林业研究国家林业局重点实验室;
【基金】：中央公益性科研院所基金项目(RITFYWZX201506;CAFYBB2016SZ002) “十三五”国家重点研发计划项目任务(2017YFD0601101-7) 林业公益性行业科研专项(201204304-02)共同资助
【分类号】：Q943.2;S792.99
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